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Anticorps > 20111 - Purified freeze-dried antibody to human type I collagen

20111 - Purified freeze-dried antibody to human type I collagen



Human skin paraffin section, IHC


species

Human



immunogen

Type I collagen




Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]

Immunogen : Type I collagen extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, WB.

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #387f
  • #471g
  • #532c

Presentation

Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]



Description

Immunogen : Type I collagen extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, WB.

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #387f
    • IgG :

      1.4 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.

  • #471g
    • IgG :

      2.79



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.

  • #532c
    • IgG :

      0.98 mg/mL



      pH :

      7.41

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.




References

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    Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D.
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    Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A.
  • «A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. J. Histochem. Cytochem. 1985, 33, 407-414.»
    Clement B., Rissel M., Peyrol S., Mazurier Y., Grimaud J.A., Guillouzo A.
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    Berrih S., Savino W., Cohen S.
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    Peyrol S., Hugues B., Grimaud J.A., Mornex R.
  • «Cytodifferentiation of human dental pulp cells in explant cultures. In : tooth morphogenesis and differentiation. Belcourt A.B., Ruch J.V. Eds. Les Editions INSERM, Paris 1984, 125, 119-126.»
    Magloire H., Hartmann D.J., Couble M.L., Joffre A., Grimaud J.A., Herbage D., Ville G.
  • «Connective tissue organization of healthy human gingiva. Ultrastructural localization of collagen types I-III-IV. J. Periodont. Res. 1984, 19, 221-229.»
    Chavrier C., Couble M.L., Magloire H., Grimaud J.A.
  • «Connective matrix organisation in human pulmonary fibrosis. Virchows Arch. [Cell. Pathol.] 1983, 44, 223-240.»
    Takiya C., Peyrol S., Cordier J.F., Grimaud J.A.
  • «Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 1982, 74, 319-328.»
    Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.