Anticorps > 20441 - Purified freeze-dried antibody to rat type IV collagen
20441 - Purified freeze-dried antibody to rat type IV collagen
Rat skin paraffin section, IHC
species
Rat
immunogen
Type IV collagen
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Immunogen : Type IV collagen extracted from rodent tumor tissues.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilution should be determined by the end user.
- #327t
-
IgG
1,0 mg/mL
pH
7,5
ELISA
Optimal working dilution at 1/6400.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen rat skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
Presentation
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Description
Immunogen : Type IV collagen extracted from rodent tumor tissues.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilution should be determined by the end user.
Lots
- #327t
-
IgG :
1,0
pH :
7,5
ELISA
Optimal working dilution at 1/6400.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen rat skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
References
- «Neurovascular Protection by Telmisartan via Reducing Neuroinflammation in Stroke-Resistant Spontaneously Hypertensive Rat Brain after Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. Dec. 2014.»
Syoichiro K., Tomoko K., Kota S., Yoshio O., Nozomi H., Toru Y., PhD, Kentaro D., Koji A.
