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Anticorps > 20441 - Purified freeze-dried antibody to rat type IV collagen

20441 - Purified freeze-dried antibody to rat type IV collagen



Rat skin paraffin section, IHC


species

Rat



immunogen

Type IV collagen




Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]

Immunogen : Type IV collagen extracted from rodent tumor tissues.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.5 mL vials. Reconstitute with 0.5 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilution should be determined by the end user.


Share your experience with this antibody in your specific application

  • #327t

Presentation

Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]



Description

Immunogen : Type IV collagen extracted from rodent tumor tissues.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.5 mL vials. Reconstitute with 0.5 mL distilled water and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilution should be determined by the end user.


Lots

  • #327t
    • IgG :

      1,0



      pH :

      7,5

      ELISA


      Optimal working dilution at 1/6400.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen rat skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.




References

  • «Neurovascular Protection by Telmisartan via Reducing Neuroinflammation in Stroke-Resistant Spontaneously Hypertensive Rat Brain after Ischemic Stroke. Journal of Stroke and Cerebrovascular Diseases. Dec. 2014.»
    Syoichiro K., Tomoko K., Kota S., Yoshio O., Nozomi H., Toru Y., PhD, Kentaro D., Koji A.