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Anticorps > 24851 - Purified freeze-dried antibody to mouse laminin

24851 - Purified freeze-dried antibody to mouse laminin



Mouse skin paraffin section, IHC


species

Mouse



immunogen

Laminin




Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. [according to RefSeq]

Immunogen : Laminin extracted from mouse EHS tumor tissues.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #301r
  • #301q
  • #505j
  • #539c

Presentation

Laminin, an extracellular protein, is a major component of the basement membrane. It is thought to mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits, alpha, beta, and gamma, which are bound to each other by disulfide bonds into a cross-shaped molecule. Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. [according to RefSeq]



Description

Immunogen : Laminin extracted from mouse EHS tumor tissues.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #301r
    • IgG :

      2,5 mg/mL



      pH :

      7,3

      ELISA


      Optimal working dilution at 1/200 000.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen mouse skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on frozen mouse skin.

  • #301q
    • IgG :

      2,5



      pH :

      7,3

      ELISA


      ELISA not tested with our current protocol.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen mouse skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluranidase pretreatment, optimal working dilution at 1/1000 on frozen mouse skin.

  • #505j
    • IgG :

      1.93 mg/mL



      pH :

      7.37

      ELISA


      Optimal working dilution at 1/70000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on frozen mouse skin.

  • #539c
    • IgG :

      3.38 mg/mL



      pH :

      7.23

      ELISA


      Optimal working dilution at 1/100 000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on frozen mouse skin.




References

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    Julliard A.K., Hartmann D.J.
  • «Involvement of laminin and its receptor in abrogation of heart graft rejection by autoreactive T cells from Trypanosoma cruzi- infected mice. J. Immunol. 1997, 159, 997-1003.»
    Silva-Barbosa S.D., Cotta De Almeida V., Riederer I., De MeIm J., Dardenne M., Bonomo A., Savino W.
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    Desmoulière A., Darby I., Monte Alto Costa A., Raccurt M., Tuchweber B., Sommer P., Gabbiani G.
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    Savino W., Silva-Barbosa S.D.
  • «Extracellular matrix components of the mouse thymus microenvironment. V. Interferon- modulates thymic epithelial cell/thymocyte interactions via extracellular matrix ligand and receptors. Cell. Immunol. 1996, 170, 235-244. {{(IVm, FNh, LNm / MO-PO)}}»
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    Gindre D., Peyrol S., Raccurt M., Sommer P., Loire. R., Grimaud J.A., Cordier J.F.
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  • «Characterization of the extracellular matrix-containing giant perivascular spaces in the NOD mouse thymus. Diabetes 1993, 42, 134-140.»
    Savino W., Carnaud C., Luan J.J., Bach J.F., Dardenne M.
  • «Thymic extracellular matrix in human malnutrition. J. Pathol. 1993, 171, 231-236.»
    Ocampo Lyra J.S.P., Madi K., Maeda C.T., Savino W.
  • «The effects of PEG on second antibody immunoprecipitation and its use in immunoassay. J. Immunoassay 1993, 14, 241-266.»
    Hartmann D.J., Grassi J., Ville G.
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    Dardenne M., Boukaiba N., Gagnerault M.C., Homo-Delarche F., Chappuis P.,Lemonnier D., Savino W.
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    Lorimier P., Mezin P., Labat Moleur F., Pinel N., Peyrol S., Stoebner P.
  • «Cell-matrix patterns in the cutaneous lesion of chromomycosis. Path. Res. Pract. 1992, 188, 894-900.»
    Esterre P., Peyrol S., Guerret S., Sainte-Marie D., Pradinaud R., Grimaud J.A.
  • «Distribution of basement membranes in primary and metastatic carcinomas of the prostate. Hum. Pathol. 1992, 23, 934-939.»
    Bonkhoff H., Wernert N., Dhom G., Remberger K.
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    Tinois E., Tiollier J., Gaucherand M., Dumas H., Tardy M., Thivolet J.
  • «Studies on the thymus in non obese diabetic mouse. I. Changes in the microenvironmental compartments. Lab. Invest. 1991, 64, 405-417.»
    Savino W., Boitard C., Bach J.F., Dardenne M.
  • «Extracellular matrix components of the mouse thymus microenvironment : Ontogenetic studies and modulation by glucocortico•ds hormones. J. Histochem. Cytochem. 1991, 39, 1539-1546.»
    Lannes-Vieira J., Dardenne M., Savino W.
  • «Extracellular matrix components of the mouse thymic microenvironment. II. In vitro modulation of basement membrane proteins by interferon- : Relationship with thymic epithelial cell proliferation. Cellular Immunology 1991, 137, 329-340.»
    Lannes-Vieira J., van der Meide P.H., Savino W.
  • «Morphological and immunocytochemical characterization of cultured rat incisor cervical epithelial cells. Archs oral Biol. 1991, 36, 737-745.»
    Farges J.C., Couble M.L., Joffre A., Hartmann D.J., Magloire H.
  • «Connective tissue changes in rheumatic heart disease. J. Submicrosc. Cytol. Pathol. 1991, 23, 213-220.»
    Dos Santos W.L., Verney R.N., De Montclos H., Veysseyre C, Carraz M., Grimaud J.A.
  • «Intra-alveolar fibrosis of idiopathic bronchiolitis obliterans-organizing pneumonia: Cell-matrix patterns. Am. J. Pathol. 1990, 137, 155-170.»
    Peyrol S., Cordier J.F., Grimaud J.A.
  • «Squamous cell carcinoma in situ developing in dermoid cyst of the ovary. Report of a case with laminin immunohistochemical staining demonstrating basement membrane integrity. Path. Res. Pract. 1989, 185, 251-254.»
    Peuchmaur M., Reynes M.
  • «Immunohistochemical localization of Type IV collagen fibronectin and laminin in the juxtaglomerular apparatus of the rat kidney. Cell. Mol. Biol. 1989, 35, 469-484. »
    Blanc-Brunat N., Mutin M., Peyrol S.
  • «Electron immunolocalization of type IV collagen, laminin and fibronectin synthesized by multilayered cells cultured from human oral epithelium. J. Periodont. Res. 1988, 23, 252-257.»
    Poly H., Couble M.L., Hartmann D.J., Faure M., Magloire H.
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  • «Distribution of the major connective matrix components of the stromal reaction in breast carcinoma. Cell. Mol. Biol. 1987, 33, 453-467.»
    Mahfouz S.M., Chevallier M., Grimaud J.A.
  • «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
    Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P.
  • «Mise en évidence immunohistochimique de la laminine au niveau du col utérin. Gynécologie 1985, 36, 9-15.»
    Peuchmaur M., Reynes M., Hartmann D.J., Galtier M., Poitout P., Ville G.
  • «Myofibroblastic stromal reaction in carcinoma of the breast : variations of collagenous matrix and structural glycoproteins. Virchows Arch. 1985, 408, 49-59.»
    Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A.
  • «Early mouse molar root development : cellular changes and distribution of fibronectin, laminin and type IV collagen. Differentiation 1985, 30, 111-122.»
    Andujar M.B., Magloire H., Hartmann D.J., Ville G., Grimaud J.A.
  • «Modifications of Hertwig's epithelial cells during developing mouse molar tooth. Ultrastructural, histochemical and immunohistochemical studies. In : tooth morphogenesis and differentiation, Belcourt A.B., Ruch J.V. Eds. Les Editions INSERM, Paris 1984, 125, 535-544.»
    Andujar M.B., Magloire H., Hartmann D.J., Grimaud J.A.
  • «Immunofluorescent localization of collagen types I, III, IV, fibronectin and laminin during morphogenesis of scales and scaleless skin in the chick embryo. Roux's Arch. Dev. Biol. 1983, 192, 205-215.»
    Mauger A., Demarchez M., Herbage D., Grimaud J.A., Druguet M., Hartmann D.J. Foidart J.M., Sengel P.
  • «Répartition du collagène, de la fibronectine et de la laminine au cours de la morphogénèse de la peau et des phanères chez l'embryon de poulet. C. R. Acad. Sc. Paris 1982, 294, 475-480.»
    Mauger A., Demarchez M., Georges D., Herbage D., Grimaud J.A., Druguet M., Hartmann D.J., Sengel P.