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Anticorps > 25011 - Purified freeze-dried antibody to human elastin

25011 - Purified freeze-dried antibody to human elastin



Human skin paraffin section, IHC


species

Human



immunogen

Elastin




Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.

Immunogen : Elastin extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA)

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

Cross-reactivity with bovine species.

  • #502d
  • #520d
  • #545b

Presentation

Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.



Description

Immunogen : Elastin extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA, SP(RIA)

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Specificity

Cross-reactivity with bovine species.


Lots

  • #502d
    • IgG :

      1.75 mg/mL



      pH :

      7.42

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.

  • #520d
    • IgG :

      1.93 mg/mL



      pH :

      7.48

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.

  • #545b
    • IgG :

      1.74mg/mL



      pH :

      7.43

      ELISA


      Optimal working dilution at 1/25 000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.




References

  • «Structural and Biomechanical Characterization of a Scaffold-Free Skin Equivalent Model via Biophysical Methods - Skin pharmacology and physiology - 2020»
    Herauda S., Delalleauc A., Houcined A., Guiraud B., Bacqueville D., Payred B., Delisled MB, Bessou-Touya S. et Damour O
  • «In situ Hybridization to evaluate the wound healing process of a bilayered living cell-based construct (Apligraf®) in a nude mice model - 2010 june, TERMIS-EU, Galway, Ireland.»
    Rousseau C. and Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 2005, 11, 723-733.»
    Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O.
  • «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
    Guerret S., Govignon E., Hartmann D.J., Ronfard V.
  • «Elastin gene deletions in Williams syndrome patients result in altered deposition of elastic fibers in skin and a subclinical dermal phenotype. Pediatric Dermatology 2000, 17, 12-20.»
    Zsolt U., Peyrol S., Plauchu H., Zabot M.T., Lebwohl M., Schilling K., Green M., Boyd C.D., Csisz?r K.
  • «Keratinocytes influence the maturation and organization of the elastin network in a skin equivalent. J. Invest Dermatol 2000, 114, 365-370.»
    Duplan-Perrat F., Damour O., Montrocher C., Peyrol S., Grenier G., Jacob M.P., Braye F.
  • «Myofibroblast in schistosomal portal fibrosis of man. Mem. Inst. Oswaldo Cruz, Rio de Janeiro 1999, 94, 87-93.»
    Andrade Z.A., Guerret S., Fernandes A.M.L.
  • «Lysyl oxidase gene expression in the stromal reaction to in situ and invasive ductal breast carcinoma. Am. J. Pathol. 1997, 150, 497-507.»
    Peyrol S., Raccurt M., Gerard F., Gleyzal C., Grimaud J.A., Sommer P.
  • «Ultrastructural localization of elastin-like immunoreactivity in the extracellular matrix around human small lymphatic vessels. Lymphology 1995, 28, 189-195.»
    Poggi P., Casasco A., Marchetti C., Casasco M., Cornaglia A., Calligaro A., Leithias C., Hartmann D.J.
  • «Cell-matrix patterns in the cutaneous lesion of chromomycosis. Path. Res. Pract. 1992, 188, 894-900.»
    Esterre P., Peyrol S., Guerret S., Sainte-Marie D., Pradinaud R., Grimaud J.A.
  • «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
    Demarchez M., Hartmann D.J., Regnier M., Asselineau D.
  • «Changes in the elastic tissue of the non-sun-exposed skin of cigarette smokers. Br. J. Dermatol. 1991, 125, 43-47.»
    Frances C., BoImnic S., Hartmann D.J., Dautzenberg B., Branchet M.C., Le Charpentier Y, Robert L.
  • «Connective tissue changes in rheumatic heart disease. J. Submicrosc. Cytol. Pathol. 1991, 23, 213-220.»
    Dos Santos W.L., Verney R.N., De Montclos H., Veysseyre C, Carraz M., Grimaud J.A.
  • «Elastin in human, baboon, and mouse liver: an immunohistochemical and immunoelectron microscopic study. Anat. Rec. 1990, 228, 392-404.»
    Porto L.C., Chevallier M., Peyrol S., Guerret S., Grimaud J.A.
  • «Elastin in alcoholic liver disease. Path. Res.Pract. 1990, 186, 668-679.»
    Porto L.C., Chevallier M., Guerret S., Hartmann D.J., Grimaud J.A.
  • «Intra-alveolar fibrosis of idiopathic bronchiolitis obliterans-organizing pneumonia: Cell-matrix patterns. Am. J. Pathol. 1990, 137, 155-170.»
    Peyrol S., Cordier J.F., Grimaud J.A.
  • «Distribution and organization of the elastic system fibres in healthy human gingiva. Ultrastructural and immunohistochemical study. Histochemistry 1988, 89, 47-52.»
    Chavrier C., Hartmann D.J., Couble M.L., Herbage D.
  • «Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 1987, 35, 15-21.»
    Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R.
  • «Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-tissue reorganization. Dev. Biol. 1987, 121, 119-129.»
    Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.