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Anticorps > 20111 - Purified freeze-dried antibody to human type I collagen

20111 - Purified freeze-dried antibody to human type I collagen



Human skin paraffin section, IHC


species

Human



immunogen

Type I collagen




Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]

Immunogen : Type I collagen extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, WB.

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #380j
  • #380k
  • #387f
  • #380o
  • #471g

Presentation

Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]



Description

Immunogen : Type I collagen extracted from human skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, WB.

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #380j
    • IgG :

      1.1 mg/mL



      pH :

      7.4

      ELISA


      Optimal working dilution at 1/4000.


      IF


      Optimal working dilution not tested


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.

  • #380k
    • IgG :

      1.1 mg/mL



      pH :

      7.4

      ELISA


      Optimal dilution at 1/4000.


      IF


      Not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #387f
    • IgG :

      1.4 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.

  • #380o
    • IgG :

      1.1 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/4000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.

  • #471g
    • IgG :

      2.79



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.




References

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    Grimbert S., Trinchet J.C., Hartmann D.J., Munz C., Garnier M., Mal F., Callard P., Beaugrand M.
  • «Thrombospondin and other possible related matrix proteins in malignant and benign breast disease. Am. J. Pathol. 1992, 140, 1473-1482.»
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  • «Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol. Clin. Exp. Res. 1992, 16, 342-346.»
    Trinchet J.C., Hartmann D.J., Pateron D., Munz-Gotheil C., Callard P., Ville G., Beaugrand M.
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  • «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
    Demarchez M., Hartmann D.J., Regnier M., Asselineau D.
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    Trinchet J.C., Hartmann D.J., Pateron D., Laarif M., Callard P., Ville G., Beaugrand M.
  • «Endothelial cell growth factor and heparin regulate collagen gene expression in keloid fibroblasts. Biochem. J. 1991, 278, 863-869.»
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  • «Odontoblast-like cytodiffentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement. Arch. oral. Biol. 1991, 36, 117-128.»
    Seux D., Couble M.L., Hartmann D.J., Gauthier J.P., Magloire H.
  • «Modulation of human dermal fibroblast extracellular matrix metabolism by the lymphokine leukoregulin. J. Cell Biol. 1991, 113, 1455-1462.»
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    Mauviel A., Heino J., Kähäri V.M., Hartmann D.J., Loyau G., Pujol J.P., Vuorio E.
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    Veron M.H., Couble M.L., Caillot G., Hartmann D.J.
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    Mauviel A., Teyton L., Bhatnagar R., PenformIm H., Laurent M., Hartmann D.J., Bonaventure J., Loyau G., Saklatvala J., Pujol J.P.
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    Andrade Z.A., Grimaud J.A.
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    Melin M., Saeland S., Magloire H., Hartmann D.J., Guerret S., Blanchard D., Banchereau J., Grimaud J.A.
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    Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P.
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    Exbrayat P., Couble M.L., Magloire H., Hartmann D.J.
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    Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A.
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    Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A.
  • «Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-tissue reorganization. Dev. Biol. 1987, 121, 119-129.»
    Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M.
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    Chomette G., Auriol M., Armbruster D., Szpirglas H., Vaillant J.M.
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    Chavrier C., Couble M.L., Hartmann D.J., Grimaud J.A., Magloire H.
  • «Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 1987, 88, 85-89.»
    Bedossa P., Bacci J., Lemaigre G., Martin E.
  • «Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus 1986, 8, 245-256.»
    Savino W., Itoh T., Imhof B.A., Dardenne M.
  • «Type-I collagen production by human odontoblast-like cells in explants cultured on cyanoacrylate films. Cell Tissue Res. 1986, 244, 133-140.»
    Magloire H., Callé A., Hartmann D.J., Joffre A., Serre B., Grimaud J.A., Schue F.
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    Lefaix J.L., Verola O., Brocheriou C., Daburon F.
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    "Boy-Lefevre M.L., Nefussi J.R., Paulin D., Hartmann D.J., Ricard Blum S., Herbage D., Forest N. "
  • «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
    Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D.
  • «Myofibroblastic stromal reaction in carcinoma of the breast : variations of collagenous matrix and structural glycoproteins. Virchows Arch. 1985, 408, 49-59.»
    Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A.
  • «A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. J. Histochem. Cytochem. 1985, 33, 407-414.»
    Clement B., Rissel M., Peyrol S., Mazurier Y., Grimaud J.A., Guillouzo A.
  • «Extracellular matrix of the human thymus : immunoflorescence studies on frozen sections and cultured epithelial cells. J. Histochem. Cytochem. 1985, 33, 655-664.»
    Berrih S., Savino W., Cohen S.
  • «Collagen heterogeneity of thickened basement membranes of human diabetic dermis : tissular immunolabelling of collagen types I, III and IV by light and electron microscopy. Cell. Mol. Biol. 1984, 30, 43-57.»
    Peyrol S., Hugues B., Grimaud J.A., Mornex R.
  • «Cytodifferentiation of human dental pulp cells in explant cultures. In : tooth morphogenesis and differentiation. Belcourt A.B., Ruch J.V. Eds. Les Editions INSERM, Paris 1984, 125, 119-126.»
    Magloire H., Hartmann D.J., Couble M.L., Joffre A., Grimaud J.A., Herbage D., Ville G.
  • «Connective tissue organization of healthy human gingiva. Ultrastructural localization of collagen types I-III-IV. J. Periodont. Res. 1984, 19, 221-229.»
    Chavrier C., Couble M.L., Magloire H., Grimaud J.A.
  • «Connective matrix organisation in human pulmonary fibrosis. Virchows Arch. [Cell. Pathol.] 1983, 44, 223-240.»
    Takiya C., Peyrol S., Cordier J.F., Grimaud J.A.
  • «Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 1982, 74, 319-328.»
    Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.