
Anticorps > 20111 - Purified freeze-dried antibody to human type I collagen
20111 - Purified freeze-dried antibody to human type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from human skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, WB.
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #387f
-
IgG
1.4 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/8000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.
-
- #471g
-
IgG
2.79
pH
7.32
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.
-
- #532c
-
IgG
0.98 mg/mL
pH
7.41
ELISA
Optimal working dilution at 1/2000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from human skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, WB.
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #387f
-
IgG :
1.4 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/8000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.
-
- #471g
-
IgG :
2.79
pH :
7.32
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.
-
- #532c
-
IgG :
0.98 mg/mL
pH :
7.41
ELISA
Optimal working dilution at 1/2000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.
-
References
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Castera L., Hartmann D.J., Chapel F., Guettier C., Mal F., Lons T., Richardet J.P., Grimbert S., Morassi O., Beaugrand M., Trinchet J.C. - «Immunohistochemical study of collagens of the extracellular matrix in cartilage of Sepia officinalis. Eur. J. Histochem. 1999, 43, 211-225.»
Bairati A., Comazzi M., Gioria M., Hartmann D.J., Leone F., Rigo C. - «Monitoring of extracellular matrix metabolism and cross-linking in tissue, serum and urine of patients with chromoblastomycosis, a chronic skin fibrosis. Eur. J. Clin. Invest. 1998, 28, 748-754.»
Ricard-Blum S., Hartmann D.J., Esterre Ph. - «Biochemical markers of bone metabolism in draught and warmblood horses. The Veterinary J., 1998, 156,169-175.»
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Cai Y., Sich M., Beziau A., Kleppel M.M., Gubler M.C. - «Fibrosing vasculitis in Wegener's granulomatosis : ultrastructural and immunohistochemical analysis of the vascular lesions. Virchows Arch. 1995, 427, 385-393.»
Gindre D., Peyrol S., Raccurt M., Sommer P., Loire. R., Grimaud J.A., Cordier J.F. - «Distribution of the extracellular matrix components in human glomerular lesions. J. Pathol. 1994, 172, 199-207.»
Büyükbabani N., Droz D. - «Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 1994, 4, 113-123.»
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Hartmann D.J., Grassi J., Ville G. - «Comparaison de 6 marqueurs sériques potentiels de la fibrose hépatique au cours de la maladie alcoolique du foie. Gastroenterol. Clin. Biol. 1993, 17, 570-577.»
Grimbert S., Trinchet J.C., Hartmann D.J., Munz C., Garnier M., Mal F., Callard P., Beaugrand M. - «Thrombospondin and other possible related matrix proteins in malignant and benign breast disease. Am. J. Pathol. 1992, 140, 1473-1482.»
Wong S.Y., Purdie A.T., Han P. - «Immunocytochemical localization of collagen types I, III, IV, and fibronectin in the human dermis. Cell Tissue Res. 1992, 268, 505-511.»
Vitellaro-Zuccarello L., Garbelli R., Dal Pozzo Rossi V. - «Serum type I collagen and N-terminal peptide of type III procollagen in patients with alcoholic liver disease: relationship to liver histology. Alcohol. Clin. Exp. Res. 1992, 16, 342-346.»
Trinchet J.C., Hartmann D.J., Pateron D., Munz-Gotheil C., Callard P., Ville G., Beaugrand M. - «Ultrastructural localization of the major components of the extracellular matrix in normal rat nerve. J. Histochem. Cytochem. 1992, 40, 859-868.»
Lorimier P., Mezin P., Labat Moleur F., Pinel N., Peyrol S., Stoebner P. - «Mesangial deposition of Type I collagen in human glomerulosclerosis. Hum. Pathol. 1992, 23, 1373-1379.»
Glick A.D., Jacobson H.R., Haralson M.A. - «Changes in the extracellular matrix of the normal human breast during the menstrual cycle. Cell Tissue Res. 1992, 268, 167-177.»
Ferguson J.E., Schor A.M., Howell A., Ferguson M.W.J. - «Cell-matrix patterns in the cutaneous lesion of chromomycosis. Path. Res. Pract. 1992, 188, 894-900.»
Esterre P., Peyrol S., Guerret S., Sainte-Marie D., Pradinaud R., Grimaud J.A. - «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
Demarchez M., Hartmann D.J., Regnier M., Asselineau D. - «Serum type I collagen and N-terminal peptide of type III procollagen in chronic hepatitis. Relationship to liver histology and conventional liver tests. J. Hepatol. 1991, 12, 139-144.»
Trinchet J.C., Hartmann D.J., Pateron D., Laarif M., Callard P., Ville G., Beaugrand M. - «Endothelial cell growth factor and heparin regulate collagen gene expression in keloid fibroblasts. Biochem. J. 1991, 278, 863-869.»
Tan E.M.L., Peltonen J. - «Odontoblast-like cytodiffentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement. Arch. oral. Biol. 1991, 36, 117-128.»
Seux D., Couble M.L., Hartmann D.J., Gauthier J.P., Magloire H. - «Modulation of human dermal fibroblast extracellular matrix metabolism by the lymphokine leukoregulin. J. Cell Biol. 1991, 113, 1455-1462.»
Mauviel A., Redini F, Hartmann D.J., Pujol J.P., Evans C.E. - «Comparative effects of interleukin-1 and tumor necrosis factor on collagen production and corresponding procollagen mRNA levels in human dermal fibroblasts. J. Invest. Dermatol. 1991, 96, 243-249.»
Mauviel A., Heino J., Kähäri V.M., Hartmann D.J., Loyau G., Pujol J.P., Vuorio E. - «Extracellular matrix components of the mouse thymus microenvironment : Ontogenetic studies and modulation by glucocorticods hormones. J. Histochem. Cytochem. 1991, 39, 1539-1546.»
Lannes-Vieira J., Dardenne M., Savino W. - «Extracellular matrix components of the mouse thymic microenvironment. II. In vitro modulation of basement membrane proteins by interferon- : Relationship with thymic epithelial cell proliferation. Cellular Immunology 1991, 137, 329-340.»
Lannes-Vieira J., van der Meide P.H., Savino W. - «Connective tissue changes in rheumatic heart disease. J. Submicrosc. Cytol. Pathol. 1991, 23, 213-220.»
Dos Santos W.L., Verney R.N., De Montclos H., Veysseyre C, Carraz M., Grimaud J.A. - «Expression of fibronectin and type I collagen by human dental-pulp cells and gingiva fibroblasts grown on fibronectin substrate. Archs. Oral Biol. 1990, 35, 565-569.»
Veron M.H., Couble M.L., Caillot G., Hartmann D.J. - «Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen. Biochim. Biophys. Acta 1990, 1038, 222-230.»
Ronzière M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D. - «Intra-alveolar fibrosis of idiopathic bronchiolitis obliterans-organizing pneumonia: Cell-matrix patterns. Am. J. Pathol. 1990, 137, 155-170.»
Peyrol S., Cordier J.F., Grimaud J.A. - «Radioimmunoassay of type I collagen that mainly detects degradation products in serum: application to patients with liver diseases. Clin. Chem. 1990, 36, 421-426.»
Hartmann D.J., Trinchet J.C., Ricard-Blum S., Beaugrand M., Callard P., Ville G. - «Human recombinant gamma-interferon stimulates proliferation and inhibits collagen and fibronectin production by human dental pulp fibroblasts. Cell. M. Biol. 1989, 35, 97-110.»
Melin M., Hartmann D.J., Magloire H., Falcoff E., Auriault C., Grimaud J.A. - «Effet des insaponifiables d'avocat/soja (PIAS) sur la production de collagène par des cultures de synoviocytes, chondrocytes articulaires et fibroblastes dermiques. Revue du Rhumatisme 1989, 56, 207-211.»
Mauviel A., Daireaux M., Hartmann D.J., Galera P., Loyau G., Pujol J.P. - «Effect of naproxen on the production of collagen by interleukin-1-stimulated skin fibroblasts, synovial-cells, and articular chondrocytes. Curr. Ther. Res. 1989, 45, 525-539.»
Galera P., Hartmann D.J., Daireaux M., Mauviel A., Jube L., Loyau G., Pujol J.P. - «Thymic extracellular matrix in Down's syndrome. Brazilian J. Med. Biol. Res. 1989, 22, 971-974.»
Fonseca E.C., Lannes-Vieira J., Villa-Verde D.M., Savino W. - «Immunohistochemical study of types I, III and IV collagen in diseased human gingiva of patients with rapidly progressive periodontitis : a light and electron microscopic study. Cell. Mol. Biol. 1989, 35, 457-467.»
Chavrier C., Couble M.L. - «Gene expression of fibroblast matrix proteins In altered by indomethacin. FEBS Lett. 1988, 231, 125-129.»
Mauviel A., Kähäri V.M., Heino J., Daireaux M., Hartmann D.J., Loyau G., Pujol J.P. - «Interleukin-1 modulates collagen gene expression in cultured synovial cells. Biochem. J. 1988, 252, 247-255.»
Mauviel A., Teyton L., Bhatnagar R., PenformIm H., Laurent M., Hartmann D.J., Bonaventure J., Loyau G., Saklatvala J., Pujol J.P. - «Glomerular distribution of subunits M1 and M2 of the globular domain of basement membrane collagen in alport's syndrome. In : Progress in basement membrane research. Renal and related aspects in health and disease, Gubler M.C., Sternberg M. Eds, J. Libbey Eurotext Ltd, Paris, 1988, 177-182.»
Gubler M.C., Mounier F., Gros F., Wieslander J., Beziau A., Guicharnaud L. - «Production de collagène de type I et III par des cellules de pulpes de dents humaines en culture : action de surnageant de macrophages stimulés par des lipopolysaccharides bactériens. Rev. Fr. Endodontie 1988, 7, 9-15.»
Bobillon G., Hartmann D.J., Joffre A., Magloire H. - «Light chain deposition disease with liver dysfunction. Hum. Pathol. 1988, 19, 1008-1014.»
Bedossa P., Favre M., Paraf F., Martin E., Lemaigre G. - «Morphology of chronic collagen resorption : a study on the late stages of schistosomal granuloma involution. Am. J. Path. 1988, 132, 389-399.»
Andrade Z.A., Grimaud J.A. - «Supernatant from an activated human CD: 4+ T-cell clone modulates the proliferation and collagen synthesis of human dental pulp fibroblasts. Collagen Rel. Res. 1987, 7, 371-381.»
Melin M., Saeland S., Magloire H., Hartmann D.J., Guerret S., Blanchard D., Banchereau J., Grimaud J.A. - «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P. - «Evaluation of the biocompatibility of a Ni-Cr-Mo dental alloy with human gingival explant culture in vitro : morphological study, immunodetection of fibronectin, and collagen production. Biomaterials 1987, 8, 385-392.»
Exbrayat P., Couble M.L., Magloire H., Hartmann D.J. - «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. - «Histo-immunolocalisation des collagènes de type I et III humains et murins de cancers humains greffés chez la souris athymique congénitale. C.R. Acad. Sc. Paris 1987, 304, 155-160.»
Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A. - «Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-tissue reorganization. Dev. Biol. 1987, 121, 119-129.»
Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M. - «La maladie périodontale de l'adulte. Etude immunohistochimique des cellules inflammatoires et du collagène. Rev. Stomatol. Chir. maxillofac. 1987, 88, 372-376.»
Chomette G., Auriol M., Armbruster D., Szpirglas H., Vaillant J.M. - «Immunohistochemical study of types I, III and IV collagen in fibrosis of diseased gingiva during chronic periodontitis : a light and electron microscopic study. J. Periodont. Res. 1987, 22, 29-36.»
Chavrier C., Couble M.L., Hartmann D.J., Grimaud J.A., Magloire H. - «Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 1987, 88, 85-89.»
Bedossa P., Bacci J., Lemaigre G., Martin E. - «Immunohistochemical studies on the phenotype of murine and human thymic stromal cell lines. Thymus 1986, 8, 245-256.»
Savino W., Itoh T., Imhof B.A., Dardenne M. - «Type-I collagen production by human odontoblast-like cells in explants cultured on cyanoacrylate films. Cell Tissue Res. 1986, 244, 133-140.»
Magloire H., Callé A., Hartmann D.J., Joffre A., Serre B., Grimaud J.A., Schue F. - «Early changes in irradiated skeletal muscle : a histo-enzymological and immuno-cytochemical study. Brit. J. Radiology 1986, sup 19, 109-113. {»
Lefaix J.L., Verola O., Brocheriou C., Daburon F. - «"Collagen expression during teratocarcinoma cell line-induced endochondral bone tumor. J. Histochem. Cytochem. 1986, 34, 835-839."»
"Boy-Lefevre M.L., Nefussi J.R., Paulin D., Hartmann D.J., Ricard Blum S., Herbage D., Forest N. " - «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D. - «Myofibroblastic stromal reaction in carcinoma of the breast : variations of collagenous matrix and structural glycoproteins. Virchows Arch. 1985, 408, 49-59.»
Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A. - «A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. J. Histochem. Cytochem. 1985, 33, 407-414.»
Clement B., Rissel M., Peyrol S., Mazurier Y., Grimaud J.A., Guillouzo A. - «Extracellular matrix of the human thymus : immunoflorescence studies on frozen sections and cultured epithelial cells. J. Histochem. Cytochem. 1985, 33, 655-664.»
Berrih S., Savino W., Cohen S. - «Collagen heterogeneity of thickened basement membranes of human diabetic dermis : tissular immunolabelling of collagen types I, III and IV by light and electron microscopy. Cell. Mol. Biol. 1984, 30, 43-57.»
Peyrol S., Hugues B., Grimaud J.A., Mornex R. - «Cytodifferentiation of human dental pulp cells in explant cultures. In : tooth morphogenesis and differentiation. Belcourt A.B., Ruch J.V. Eds. Les Editions INSERM, Paris 1984, 125, 119-126.»
Magloire H., Hartmann D.J., Couble M.L., Joffre A., Grimaud J.A., Herbage D., Ville G. - «Connective tissue organization of healthy human gingiva. Ultrastructural localization of collagen types I-III-IV. J. Periodont. Res. 1984, 19, 221-229.»
Chavrier C., Couble M.L., Magloire H., Grimaud J.A. - «Connective matrix organisation in human pulmonary fibrosis. Virchows Arch. [Cell. Pathol.] 1983, 44, 223-240.»
Takiya C., Peyrol S., Cordier J.F., Grimaud J.A. - «Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 1982, 74, 319-328.»
Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.