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Anticorps > 20511 - Purified freeze-dried antibody to human type V collagen

20511 - Purified freeze-dried antibody to human type V collagen



Human skin paraffin section, IHC


species

Human



immunogen

Type V collagen




Type V collagen is a fibrillar collagen composed of α chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [according to RefSeq]

Immunogen : Type V collagen extracted from human placenta.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA ,IB/WB, SP(RIA)

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

Cross-reactivity with bovine and porcine species.

  • #317j

    • IgG

      4,2 mg/mL



      pH

      7,6

      ELISA


      Optimal working dilution at 1/3200.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at optimal working dilution at 1/80 on frozen human skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #523a

    • IgG

      3.46 mg/mL



      pH

      7.59

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
  • #317m

    • IgG

      3.04



      pH

      7.45

      ELISA


      Optimal working dilution at 1/7000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #508d

    • IgG

      2.69 mg/mL



      pH

      7.30

      ELISA


      Optimal working dilution at 1/5000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
  • #524e

    • IgG

      2.57 mg/mL



      pH

      7.37

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #538c

    • IgG

      2.71mg/mL



      pH

      7.2

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.

Presentation

Type V collagen is a fibrillar collagen composed of α chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [according to RefSeq]



Description

Immunogen : Type V collagen extracted from human placenta.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA ,IB/WB, SP(RIA)

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Specificity

Cross-reactivity with bovine and porcine species.


Lots

  • #317j

    • IgG :

      4,2



      pH :

      7,6

      ELISA


      Optimal working dilution at 1/3200.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at optimal working dilution at 1/80 on frozen human skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #523a

    • IgG :

      3.46 mg/mL



      pH :

      7.59

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
  • #317m

    • IgG :

      3.04



      pH :

      7.45

      ELISA


      Optimal working dilution at 1/7000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #508d

    • IgG :

      2.69 mg/mL



      pH :

      7.30

      ELISA


      Optimal working dilution at 1/5000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
  • #524e

    • IgG :

      2.57 mg/mL



      pH :

      7.37

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
  • #538c

    • IgG :

      2.71mg/mL



      pH :

      7.2

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.



References

  • «Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. 2014 Jun;15(2):267-75.»
    Laurent R., Nallet A., Obert L., Nicod L., Gindraux F.
  • «In situ Hybridization to evaluate the wound healing process of a bilayered living cell-based construct (Apligraf®) in a nude mice model - 2010 june, TERMIS-EU, Galway, Ireland.»
    Rousseau C. and Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 2005, 11, 723-733.»
    Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O.
  • «Control of heterotypic fibril formation by collagen V is determined by chain stoichiometry. J. Biol. Chem. 2001, 276, 24352-24359.»
    Chanut-Delalande H., Fichard A., Bernocco S., Garrone R., Hulmes D.J.S., Ruggiero F.
  • «Type V regulates the assembly of collagen fibrils in cultures of bovine vascular smooth muscle cells. J. Cell. Biochem. 2000, 80, 146-155.»
    Kypreos K.E., Birk D., Trinkaus-Randall V., Hartmann D.J., Sonenshein G.E.
  • «Distribution of extracellular matrix components in the developing ruminant corpus luteum: a wound repair hypothesis for luteinisation. J. Reproduction and Fertility 1999, 116, 187-198.»
    Silvester L.M., Luck M.R.
  • «Transforming growth factor §1 stimulates type V collagen expression in bovine vascular smooth muscle cells. J. Biol. Chem. 1994, 269, 9603-9609.»
    Lawrence R., Hartmann D.J., Sonenshein G.E.
  • «The human rhabdomyosarcoma cell line A204 lays down a highly insoluble matrix composed mainly of 1 type-XI and 2 type-V collagen chains. Eur. J. Biochem. 1992, 210, 329-335.»
    Kleman J.P., Hartmann D.J., Ramirez F., Van der Rest M.
  • «Intra-alveolar fibrosis of idiopathic bronchiolitis obliterans-organizing pneumonia: Cell-matrix patterns. Am. J. Pathol. 1990, 137, 155-170.»
    Peyrol S., Cordier J.F., Grimaud J.A.
  • «Connective matrix organisation in human pulmonary fibrosis. Virchows Arch. [Cell. Pathol.] 1983, 44, 223-240.»
    Takiya C., Peyrol S., Cordier J.F., Grimaud J.A.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.