Anticorps > 20121 - Purified freeze-dried antibody to bovine type I collagen
20121 - Purified freeze-dried antibody to bovine type I collagen
Bovine skin paraffin section, IHC
species
Bovine
immunogen
Type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #322l
-
IgG
2.83 mg/mL
pH
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #322m
-
IgG
2.95
pH
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #521a
-
IgG
2.91 mg/mL
pH
7.51
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine skin.
-
- #533d
-
IgG
2.90 mg/mL
pH
7.23
ELISA
Optimal working dilution at 1/7000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #322l
-
IgG :
2.83 mg/mL
pH :
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #322m
-
IgG :
2.95
pH :
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #521a
-
IgG :
2.91 mg/mL
pH :
7.51
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine skin.
-
- #533d
-
IgG :
2.90 mg/mL
pH :
7.23
ELISA
Optimal working dilution at 1/7000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
References
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Perris R., Löfberg J., Fällström C., Von Boxberg Y., Olsson L., Newgreen D.F. - «Physiological behaviour of rabbit articular chondrocytes immobilized in a calcium alginate gel. In : Physiology of immobilized cells, J.A.M.de Bont, Tramper J., Hamer G., Harder W., Mattiasson B. Eds, Elsevier Science PublImhers B.V., Amsterdam, 1990.»
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Vialle-Presles M.J., Hartmann D.J., Franc S., Herbage D. - «Rabbit articular chondrocytes : an in vitro model for studying the effect of sodium auro-thiopropanol sulfonate on proliferation kinectics, type II collagen phenotype and mitochondrial activity. Fundam. Clin.Pharmacol. 1988, 2,57-67.»
Ronot X., Hainque B., ChrImten M.O., Froger B., Hartmann D.J., Adolphe M., Lechat P. - «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D. - «Biochemical properties and immunolocalization of minor collagens in foetal calf cartilage. FEBS Letters 1982, 146, 343-347.»
Ricard-Blum S., Hartmann D.J., Herbage D., Payen-Meyran C., Ville G.
