Anticorps > 20121 - Purified freeze-dried antibody to bovine type I collagen
20121 - Purified freeze-dried antibody to bovine type I collagen
Bovine skin paraffin section, IHC
species
Bovine
immunogen
Type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #322m
-
IgG
2.95
pH
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #521a
-
IgG
2.91 mg/mL
pH
7.51
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine skin.
-
- #533d
-
IgG
2.90 mg/mL
pH
7.23
ELISA
Optimal working dilution at 1/7000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #322m
-
IgG :
2.95
pH :
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
- #521a
-
IgG :
2.91 mg/mL
pH :
7.51
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine skin.
-
- #533d
-
IgG :
2.90 mg/mL
pH :
7.23
ELISA
Optimal working dilution at 1/7000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.
-
References
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Ronot X., Hainque B., ChrImten M.O., Froger B., Hartmann D.J., Adolphe M., Lechat P. - «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
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Ricard-Blum S., Hartmann D.J., Herbage D., Payen-Meyran C., Ville G.
