20531 - Purified freeze-dried antibody to chicken type V collagen

Type V collagen is a fibrillar collagen composed of α chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. [according to RefSeq]

Immunogen : Type V collagen extracted from chicken skin.

Host : Rabbit.

Polyclonal antibody purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

Cross-reactivity with bovine type V collagen.

• #255

  • IgG : not tested pH : not tested

    ELISA Optimal working dilution not tested with our current protocol. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen chicken skin. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded chicken skin.

• #468c

  • IgG : 1.73 pH : 8.84

    ELISA Optimal working dilution at 1/10 000. IF ELISA Optimal working dilution at 1/7000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin. ELISA Optimal working dilution not tested with our current protocol. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 with frozen human skin. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin. #295o #297p #297s IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded chicken skin.