20611 - Purified freeze-dried antibody to human type VI collagen

Type VI Collagen is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. [according to RefSeq]

Immunogen : Type VI collagen extracted from human placenta.

Host : Rabbit.

Polyclonal antibody purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #358r

  • IgG : 1.6 mg/mL pH : 7.4

    ELISA Optimal working dilution at 1/8000. IF Not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.

• #531d

  • IgG : 1.9 mg/mL pH : 7.47

    ELISA Optimal working dilution at 1/8000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.