24921 - Purified freeze-dried antibody to bovine fibronectin

Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]

Immunogen : Fibronectin extracted from bovine plasma.

Host : Rabbit.

Polyclonal antibody purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #241

  • IgG : not tested pH : not tested

    ELISA Optimal working dilution not tested with our current protocol. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5 hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.

• #438d

  • IgG : 2,795 pH : 7.38

    ELISA Optimal working dilution at 1/50000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded bovine skin.