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Anticorps > 24941 - Purified freeze-dried antibody to rat fibronectin

24941 - Purified freeze-dried antibody to rat fibronectin



Rat skin paraffin section, IHC


species

Rat



immunogen

Fibronectin




Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]

Immunogen : Fibronectin extracted from rat plasma.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #303k
  • #303j

Presentation

Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]



Description

Immunogen : Fibronectin extracted from rat plasma.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #303k
    • IgG :

      2,3



      pH :

      7,5

      ELISA


      Optimal working dilution not tested with pur current protocol.


      IF


      Optimal working dilution not tested.


      IHC


      Optimal working dilution not tested.

  • #303j
    • IgG :

      2.3 mg/mL



      pH :

      7.5

      ELISA


      Optimal working dilution not tested with our current protocol.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 with frozen salmon skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.