Anticorps > 20131 - Purified freeze-dried antibody to chicken type I collagen
20131 - Purified freeze-dried antibody to chicken type I collagen
Chicken skin paraffin section, IHC
species
Chicken
immunogen
Type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from chicken skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #325d
-
IgG
2,7 mg/mL
pH
7,6
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 on frozen chicken skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded chicken skin.
-
- #325e
-
IgG
1.29
pH
7.4
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded chicken skin.
-
- #492d
-
IgG
2.17 mg/mL
pH
7.44
ELISA
Optimal working dilution at 1/50000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded chicken skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from chicken skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #325d
-
IgG :
2,7
pH :
7,6
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 on frozen chicken skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded chicken skin.
-
- #325e
-
IgG :
1.29
pH :
7.4
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded chicken skin.
-
- #492d
-
IgG :
2.17 mg/mL
pH :
7.44
ELISA
Optimal working dilution at 1/50000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded chicken skin.
-
References
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Rigo C., Hartmann D.J., Bairati A. - «Immunohistochemical study of collagens of the extracellular matrix in cartilage of Sepia officinalis. Eur. J. Histochem. 1999, 43, 211-225.»
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Mallein-Gerin F., Ruggiero F., Garrone R. - «Production of fibronectin and collagen types I and III by chick embryo dermal cells cultured on extracellular matrix substrates. Int. J. Dev. Biol. 1989, 33, 267-275.»
Robert J., Hartmann D.J., Sengel P. - «Influence of various extracellular matrix components on the behavior of cultured chick embryo dermal cells. Int. J. Dev. Biol. 1989, 33, 227-237.»
Robert J., Mauger A., Sengel P. - «In vitro morphogenesis of chick embryo hypertrophic cartilage. J. Cell. Biol., 1987, 105, 999-1006.»
Tacchetti C., Quarto R., Nitsch L., Hartmann D.J., Cancedda R. - «Distribution of laminin and collagens during avian neural crest development. Development 1987, 101, 461-478.»
Duband J.L., Thiery J.P. - «Immunofluorescent localization of collagen types I, III, IV, fibronectin and laminin during morphogenesis of scales and scaleless skin in the chick embryo. Roux's Arch. Dev. Biol. 1983, 192, 205-215.»
Mauger A., Demarchez M., Herbage D., Grimaud J.A., Druguet M., Hartmann D.J. Foidart J.M., Sengel P. - «Immunofluorescent localization of collagen types I and III, and of fibronectin during feather morphogenesis in the chick embryo. Dev. Biol. 1982, 94, 93-105.»
Mauger A., Demarchez M., Herbage D., Grimaud J.A., Druguet M., Hartmann D., Sengel P. - «Répartition du collagène, de la fibronectine et de la laminine au cours de la morphogénèse de la peau et des phanères chez l'embryon de poulet. C. R. Acad. Sc. Paris 1982, 294, 475-480.»
Mauger A., Demarchez M., Georges D., Herbage D., Grimaud J.A., Druguet M., Hartmann D.J., Sengel P.
