Anticorps > 25013 - Purified freeze-dried antibody to human elastin
25013 - Purified freeze-dried antibody to human elastin
Human skin paraffin section, IHC
species
Human
immunogen
Elastin
Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.
Immunogen : Elastin extracted from Human skin.
Host : Guinea Pig.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
- #310h
-
IgG
4,2 mg/mL
pH
7,5
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-guinea pig IgG conjugate, optimal working dilution at 1/40 on frozen human skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
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Presentation
Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.
Description
Immunogen : Elastin extracted from Human skin.
Host : Guinea Pig.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
Lots
- #310h
-
IgG :
4,2
pH :
7,5
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-guinea pig IgG conjugate, optimal working dilution at 1/40 on frozen human skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
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