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Anticorps > 25041 - Purified freeze-dried antibody to rat elastin

25041 - Purified freeze-dried antibody to rat elastin



Marquage de l'lastine dans une peau de rat.


species

Rat



immunogen

Elastin




Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.

Immunogen : Elastin extracted from rat aorta.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

Cross-reactivity with mouse elastin.

  • #333j
  • #333n
  • #478h
  • #495e

Presentation

Elastin is a key Extracellular Matrix protein that is at the origin of the elasticity and resilience of many vertebrate tissues (large arteries, lung, ligament, tendon, skin and elastic cartilage). Elastin is formed through the lysine mediated crosslinking of soluble precursor tropoelastin.



Description

Immunogen : Elastin extracted from rat aorta.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Specificity

Cross-reactivity with mouse elastin.


Lots

  • #333j
    • IgG :

      2.8 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.

  • #333n
    • IgG :

      2,58



      pH :

      7,42

      ELISA


      Optimal working dilution at 1/7000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded rat skin.

  • #478h
    • IgG :

      2.49



      pH :

      7.38

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded rat skin.

  • #495e
    • IgG :

      2.10



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded rat skin.




References

  • «Extracellular Matrix Deposition, lysyl oxidase expression, and myofibroblastic differentiation during the initial stages of cholestatic fibrosis in the rat. Lab. Invest. 1997, 76, 765-778.»
    Desmoulière A., Darby I., Monte Alto Costa A., Raccurt M., Tuchweber B., Sommer P., Gabbiani G.
  • «Elastogenesis in rat arterial grafts : elastin deposits on microfibrillar and non-microfibrillar structures. Histochem. J. 1988, 20, 715-721.»
    Lethias C., Chignier E., Garrone R., Hartmann D.J., Eloy R.
  • «Immunolabelling of freeze-fractured tissue : thin sections of rat aorta after labelling of elestic laminae. Eur. J. Cell. Biol. 1987, 44, 90-92.»
    Ruggiero F., Lethias C., Garrone R.
  • «Ultrastructural immunolocalization of elastic fibers in rat blood vessels using the protein A-gold technique. J. Histochem. Cytochem. 1987, 35, 15-21.»
    Lethias C., Hartmann D.J., Masmejean M., Ravazzola M., Sabbagh I., Ville G., Herbage D., Eloy R.