retour aux résultats Commander cet anticorps Imprimer la fiche Printer


Anticorps > 60315 - Purified freeze-dried antibody to human type III collagen

60315 - Purified freeze-dried antibody to human type III collagen



Human skin paraffin section, IHC


species

Human



immunogen

Type III collagen




Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]

Immunogen : Purified type III collagen extracted from human placenta. 

Source material : Mouse (Nude strain) ascites.

Isotype : IgG1k

 

Monoclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1mL vials. Reconstitute with 0.1mL distilled water  and store at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF  ≥ 1/40  

IHC ≥ 1/500

ELISA ≥ 1/2000  (OD = 0.5)

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

8D1-8C7

  • #316f
  • #454g

Presentation

Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]



Description

Immunogen : Purified type III collagen extracted from human placenta. 

Source material : Mouse (Nude strain) ascites.

Isotype : IgG1k

 

Monoclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1mL vials. Reconstitute with 0.1mL distilled water  and store at -20°C.


Stability

Unreconstituted

24 months at -20°C.

Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF  ≥ 1/40  

IHC ≥ 1/500

ELISA ≥ 1/2000  (OD = 0.5)

Optimal dilutions should be determined by the end user.


Clone

8D1-8C7


Lots

  • #316f
    • IgG :

      0,4



      pH :

      7,4

      ELISA


      Optimal working dilution at 1/16000.


      IF


      With fluorescein anti-mouse IgG conjugate, optimal working dilution at 1/320 on frozen human skin.


      IHC


      Immunoperoxidase technique with the Envision (mouse) / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded human skin.

  • #454g
    • IgG :

      0.78



      pH :

      7.66

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded human skin.




References

  • «In situ Hybridization to evaluate the wound healing process of a bilayered living cell-based construct (Apligraf®) in a nude mice model - 2010 june, TERMIS-EU, Galway, Ireland.»
    Rousseau C. and Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.»
    Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
    Guerret S., Govignon E., Hartmann D.J., Ronfard V.
  • «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
    Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P.
  • «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
    Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A.
  • «Histo-immunolocalisation des collagènes de type I et III humains et murins de cancers humains greffés chez la souris athymique congénitale. C.R. Acad. Sc. Paris 1987, 304, 155-160.»
    Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A.