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Anticorps > 20171S - Purified freeze-dried antibody to salmon type I collagen

20171S - Purified freeze-dried antibody to salmon type I collagen



Salmon muscle paraffin section, IHC


species

Fishes



immunogen

Type I collagen




Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…)

Immunogen : Type I collagen extracted from salmon fish skin.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #302t
  • #302u
  • #302y
  • #453k

Presentation

Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…)



Description

Immunogen : Type I collagen extracted from salmon fish skin.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Lots

  • #302t
    • IgG :

      3,1



      pH :

      7,4

      ELISA


      Optimal working dilution not tested.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 with frozen salmon skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/1000 with fixed paraffin-embedded salmon fish skin.

  • #302u
    • IgG :

      3,1



      pH :

      7,4

      ELISA


      Optimal working dilution not tested.


      IF


      With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 with frozen salmon skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/1000 with fixed paraffin-embedded salmon fish skin.

  • #302y
    • IgG :

      2.35



      pH :

      7.25

      ELISA


      Optimal working dilution at 1/3000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded salmon fish skin.

  • #453k
    • IgG :

      3.25



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/4000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded salmon muscle.