Anticorps > 27171-1 - Purified freeze-dried antibody to salmon type I collagen
27171-1 - Purified freeze-dried antibody to salmon type I collagen
Salmon muscle paraffin section, IHC
species
Fishes
immunogen
Type I collagen
Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…)
Immunogen : Type I collagen extracted from salmon fish skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
- #302u
-
IgG
3,1 mg/mL
pH
7,4
ELISA
Optimal working dilution not tested.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 with frozen salmon skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/1000 with fixed paraffin-embedded salmon fish skin.
-
- #302y
-
IgG
2.35
pH
7.25
ELISA
Optimal working dilution at 1/3000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded salmon fish skin.
-
- #453k
-
IgG
3.25
pH
7.32
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded salmon muscle.
-
Presentation
Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…)
Description
Immunogen : Type I collagen extracted from salmon fish skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
Lots
- #302u
-
IgG :
3,1
pH :
7,4
ELISA
Optimal working dilution not tested.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/80 with frozen salmon skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/1000 with fixed paraffin-embedded salmon fish skin.
-
- #302y
-
IgG :
2.35
pH :
7.25
ELISA
Optimal working dilution at 1/3000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded salmon fish skin.
-
- #453k
-
IgG :
3.25
pH :
7.32
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded salmon muscle.
-
