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Anticorps > 20311 - Purified freeze-dried antibody to human type III collagen

20311 - Purified freeze-dried antibody to human type III collagen



Human skin paraffin section, IHC


species

Human



immunogen

Type III collagen




Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]

Immunogen : Type III collagen extracted from human placenta.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.


Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA,  IB/WB, SP(RIA).


Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #400i
  • #494e

Presentation

Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]



Description

Immunogen : Type III collagen extracted from human placenta.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.


Reconstituted 

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA,  IB/WB, SP(RIA).


Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #400i
    • IgG :

      0.9



      pH :

      7,43

      ELISA


      Optimal working dilution at 1/1000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5 hyaluronidase pretreatment, optimal working dilution at 1/1000 on paraffin-embedded human skin.

  • #494e
    • IgG :

      8.38



      pH :

      8.18

      ELISA


      Optimal working dilution at 1/3000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.




References

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    Wong S.Y., Purdie A.T., Han P.
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    Dos Santos W.L., Verney R.N., De Montclos H., Veysseyre C, Carraz M., Grimaud J.A.
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    Ronzière M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D.
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    Peyrol S., Cordier J.F., Grimaud J.A.
  • «Dermal collagen fibrils are hybrids of Type I and Type III collagen molecules. J. Ultrastruct. Res. 1990, 105, 162-169.»
    Fleischmajer R., MacDonald E.D., PerlImh J.S., Burgeson R.E., FImher L.W.
  • «Human recombinant gamma-interferon stimulates proliferation and inhibits collagen and fibronectin production by human dental pulp fibroblasts. Cell. M. Biol. 1989, 35, 97-110.»
    Melin M., Hartmann D.J., Magloire H., Falcoff E., Auriault C., Grimaud J.A.
  • «Effet des insaponifiables d'avocat/soja (PIAS) sur la production de collagène par des cultures de synoviocytes, chondrocytes articulaires et fibroblastes dermiques. Revue du Rhumatisme 1989, 56, 207-211.»
    Mauviel A., Daireaux M., Hartmann D.J., Galera P., Loyau G., Pujol J.P.
  • «Effect of naproxen on the production of collagen by interleukin-1-stimulated skin fibroblasts, synovial-cells, and articular chondrocytes. Curr. Ther. Res. 1989, 45, 525-539.»
    Galera P., Hartmann D.J., Daireaux M., Mauviel A., Jube L., Loyau G., Pujol J.P.
  • «Immunohistochemical study of types I, III and IV collagen in diseased human gingiva of patients with rapidly progressive periodontitis : a light and electron microscopic study. Cell. Mol. Biol. 1989, 35, 457-467.»
    Chavrier C., Couble M.L.
  • «Gene expression of fibroblast matrix proteins In altered by indomethacin. FEBS Lett. 1988, 231, 125-129.»
    Mauviel A., Kähäri V.M., Heino J., Daireaux M., Hartmann D.J., Loyau G., Pujol J.P.
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    Mauviel A., Teyton L., Bhatnagar R., PenformIm H., Laurent M., Hartmann D.J., Bonaventure J., Loyau G., Saklatvala J., Pujol J.P.
  • «Localization and synthesis of type III collagen and fibronectin in human reparative dentine. Immunoperoxidase and immunogold staining. Histochemistry 1988, 88, 141-149.»
    Magloire H., Joffre A., Hartmann D.J.
  • «Production de collagène de type I et III par des cellules de pulpes de dents humaines en culture : action de surnageant de macrophages stimulés par des lipopolysaccharides bactériens. Rev. Fr. Endodontie 1988, 7, 9-15.»
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  • «Light chain deposition disease with liver dysfunction. Hum. Pathol. 1988, 19, 1008-1014.»
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  • «Morphology of chronic collagen resorption : a study on the late stages of schistosomal granuloma involution. Am. J. Path. 1988, 132, 389-399.»
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  • «Distribution of the major connective matrix components of the stromal reaction in breast carcinoma. Cell. Mol. Biol. 1987, 33, 453-467.»
    Mahfouz S.M., Chevallier M., Grimaud J.A.
  • «Evaluation of the biocompatibility of a Ni-Cr-Mo dental alloy with human gingival explant culture in vitro : morphological study, immunodetection of fibronectin, and collagen production. Biomaterials 1987, 8, 385-392.»
    Exbrayat P., Couble M.L., Magloire H., Hartmann D.J.
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    Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A.
  • «La maladie périodontale de l'adulte. Etude immunohistochimique des cellules inflammatoires et du collagène. Rev. Stomatol. Chir. maxillofac. 1987, 88, 372-376.»
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    Chavrier C., Couble M.L., Hartmann D.J., Grimaud J.A., Magloire H.
  • «Effects of fixation and processing on the immunohistochemical visualisation of type-I, -III and -IV collagen in paraffin-embedded liver tissue. Histochemistry 1987, 88, 85-89.»
    Bedossa P., Bacci J., Lemaigre G., Martin E.
  • «Myofibroblastic stromal reaction in carcinoma of the breast : variations of collagenous matrix and structural glycoproteins. Virchows Arch. 1985, 408, 49-59.»
    Lagacé R., Grimaud J.A., Schürch W., Seemayer T.A.
  • «A procedure for light and electron microscopic intracellular immunolocalization of collagen and fibronectin in rat liver. J. Histochem. Cytochem. 1985, 33, 407-414.»
    Clement B., Rissel M., Peyrol S., Mazurier Y., Grimaud J.A., Guillouzo A.
  • «Extracellular matrix of the human thymus : immunoflorescence studies on frozen sections and cultured epithelial cells. J. Histochem. Cytochem. 1985, 33, 655-664.»
    Berrih S., Savino W., Cohen S.
  • «Collagen heterogeneity of thickened basement membranes of human diabetic dermis : tissular immunolabelling of collagen types I, III and IV by light and electron microscopy. Cell. Mol. Biol. 1984, 30, 43-57.»
    Peyrol S., Hugues B., Grimaud J.A., Mornex R.
  • «Cytodifferentiation of human dental pulp cells in explant cultures. In : tooth morphogenesis and differentiation. Belcourt A.B., Ruch J.V. Eds. Les Editions INSERM, Paris 1984, 125, 119-126.»
    Magloire H., Hartmann D.J., Couble M.L., Joffre A., Grimaud J.A., Herbage D., Ville G.
  • «Connective tissue organization of healthy human gingiva. Ultrastructural localization of collagen types I-III-IV. J. Periodont. Res. 1984, 19, 221-229.»
    Chavrier C., Couble M.L., Magloire H., Grimaud J.A.
  • «Connective matrix organisation in human pulmonary fibrosis. Virchows Arch. [Cell. Pathol.] 1983, 44, 223-240.»
    Takiya C., Peyrol S., Cordier J.F., Grimaud J.A.
  • «Distribution of type III collagen in the pulp parenchyma of the human developing tooth. Light and electron microscope immunotyping. Histochemistry 1982, 74, 319-328.»
    Magloire H., Joffre A., Grimaud J.A., Herbage D., Couble M.L., Chavrier C.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.