retour aux résultats Commander cet anticorps Imprimer la fiche Printer


Anticorps > 20151 - Purified freeze-dried antibody to mouse type I collagen

20151 - Purified freeze-dried antibody to mouse type I collagen



Mouse skin paraffin section


species

Mouse



immunogen

Type I collagen




Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]

Immunogen : Type I collagen extracted from mouse skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #420d
  • #430i
  • #437g
  • #441h
  • #442g
  • #459g
  • #457c
  • #474e
  • #470g
  • #479f
  • #476g
  • #493i
  • #496c
  • #497j
  • #514c
  • #510d
  • #497k
  • #517j

Presentation

Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]



Description

Immunogen : Type I collagen extracted from mouse skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #420d
    • IgG :

      2.6 mg/mL



      pH :

      7.34

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #430i
    • IgG :

      2.7 mg/mL



      pH :

      7.42

      ELISA


      Optimal working dilution at 1/30 000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #437g
    • IgG :

      2.62 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #441h
    • IgG :

      3.07 mg/mL



      pH :

      7.41

      ELISA


      Optimal working dilution at 1/40000.


      IF


      Not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #442g
    • IgG :

      2.03 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #459g
    • IgG :

      2.91 mg/mL



      pH :

      7.24

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #457c
    • IgG :

      2.25 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #474e
    • IgG :

      4.7 mg/mL



      pH :

      7.30

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin

  • #470g
    • IgG :

      5.45 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/25000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #479f
    • IgG :

      6.06 mg/mL



      pH :

      7.31

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #476g
    • IgG :

      5.4 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/25000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #493i
    • IgG :

      3.73 mg/mL



      pH :

      7.26

      ELISA


      Optimal working dilution at 1/20 000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #496c
    • IgG :

      3.9 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #497j
    • IgG :

      1.76 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/15000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #514c
    • IgG :

      1.71 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #510d
    • IgG :

      2.17 mg/mL



      pH :

      7.31

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #497k
    • IgG :

      1.91 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #517j
    • IgG :

      4.37



      pH :

      7.25

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.




References

  • «Combination therapy using molecular-targeted drugs modulates tumor microenvironment and impairs tumor growth in renal cell carcinoma - Cancer Médecine _ August 2017»
    Hiroyuki Kitano, Yasuhiko Kitadai, Jun Teishima, Ryo Yuge, Shunsuke Shinmei, Keisuke Goto, Shogo Inoue, Tetsutaro Hayashi, Kazuhiro Sentani, Wataru Yasui, Akio Matsubara
  • «Transforming growth factor-?-induced CUX1 isoforms are associated with fibrosis insystemic sclerosis lung fibroblasts - Data Brief. - 2016 Aug, 10;8:1377-8. »
    Ikeda T., Fragiadaki M., Shi-Wen X., Ponticos M., Khan K., Denton C., Garcia P., Bou-Gharios G., Yamakawa A., Morimoto C., Abraham D.
  • «"Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-�, ñ1. FEBS Letters 2003, 545, 115-119."»
    Valcourt U., Gouttenoire J., Aubert-Foucher E., Herbage D., Mallein-Gérin F
  • «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
    Guerret S., Govignon E., Hartmann D.J., Ronfard V.
  • «Extracellular matrix components of the mouse thymus microenvironment. V. Interferon- modulates thymic epithelial cell/thymocyte interactions via extracellular matrix ligand and receptors. Cell. Immunol. 1996, 170, 235-244. {{(IVm, FNh, LNm / MO-PO)}}»
    Lagrota-Candido J.M., Villa-Verde D.M.S., Vanderlei F.H., Savino W.
  • «Increased deposition of extracellular matrix components in the thymus gland of MDX mouse: correlation with the muscular lesion. J. Neuroimmunology 1995, 59, 9-18.»
    Quirico-Santos T., Ribeiro M.M., Savino W.
  • «Altered deposition of extracellular matrix components in the skeletal muscle and lymph node of the MDX dystrophic mouse. Brazilian J. Med. Biol. Res. 1994, 27, 2229-2240.»
    Seixas S.I.L., Wajsenzon I.J., Savino W., Quirico-Santos T.
  • «Host-Donor interactions in healing of human split-thickness skin grafts onto nude mice: in situ hybridization, immunohistochemical, and histochemical studies. Transplantation 1992, 53, 1002-1010. »
    Plenat F., Vignaud J.M., Guerret-Stocker S., Hartmann D., Duprez K., Duprez A.
  • «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
    Demarchez M., Hartmann D.J., Regnier M., Asselineau D.
  • «Effects of dentin proteins, transforming growth factor §1 (TGF§1) and bone morphogenetic protein 2 (BMP2) on the differentiation of odontoblast in vitro. Int. J. Dev. Biol. 1992, 36, 491-503.»
    Begue-Kirn C., Smith A.J., Ruch J.V., Wozney J.M., Purchio A., Hartmann D.J., Lesot H.
  • «Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection. Differentiation 1991, 46, 89-95.»
    Bolmont C., Andujar M., Peyrol S., Grimaud J.A.
  • «Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 1988, 88, 131-140.»
    Andujar M.B., Hartmann D.J., Emonard H., Magloire H.
  • «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
    Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P.
  • «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
    Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A.
  • «Histo-immunolocalisation des collagènes de type I et III humains et murins de cancers humains greffés chez la souris athymique congénitale. C.R. Acad. Sc. Paris 1987, 304, 155-160.»
    Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A.
  • «Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-tissue reorganization. Dev. Biol. 1987, 121, 119-129.»
    Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
  • «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
    Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.