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Anticorps > 20151 - Purified freeze-dried antibody to mouse type I collagen

20151 - Purified freeze-dried antibody to mouse type I collagen



Mouse skin paraffin section


species

Mouse



immunogen

Type I collagen




Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]

Immunogen : Type I collagen extracted from mouse skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #441h
  • #459g
  • #457c
  • #474e
  • #470g
  • #476g
  • #496c
  • #497j
  • #514c
  • #510d
  • #497k
  • #517j
  • #516k
  • #537c
  • #537c
  • #543c
  • #551d
  • #554c

Presentation

Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]



Description

Immunogen : Type I collagen extracted from mouse skin.

Host : Rabbit.

 

Polyclonal antibody purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #441h
    • IgG :

      3.07 mg/mL



      pH :

      7.41

      ELISA


      Optimal working dilution at 1/40000.


      IF


      Not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #459g
    • IgG :

      2.91 mg/mL



      pH :

      7.24

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #457c
    • IgG :

      2.25 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/30000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #474e
    • IgG :

      4.7 mg/mL



      pH :

      7.30

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin

  • #470g
    • IgG :

      5.45 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/25000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #476g
    • IgG :

      5.4 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/25000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #496c
    • IgG :

      3.9 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #497j
    • IgG :

      1.76 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/15000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #514c
    • IgG :

      1.71 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/8000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #510d
    • IgG :

      2.17 mg/mL



      pH :

      7.31

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #497k
    • IgG :

      1.91 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #517j
    • IgG :

      4.37



      pH :

      7.25

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #516k
    • IgG :

      4.48 mg/mL



      pH :

      7.39

      ELISA


      Optimal working dilution at 1/10000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

  • #537c
    • IgG :

      2.08 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/30 000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #537c
    • IgG :

      2.08 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/30 000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.

  • #543c
    • IgG :

      4.65mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/20000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #551d
    • IgG :

      5.07mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/5000.


      IF


      Optimal working dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.

  • #554c
    • IgG :

      4.26



      pH :

      7.31

      ELISA


      Optimal working dilution at 1/10000


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.




References

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  • «"Alternative splicing of type II procollagen pre-mRNA in chondrocytes is oppositely regulated by BMP-2 and TGF-�, ñ1. FEBS Letters 2003, 545, 115-119."»
    Valcourt U., Gouttenoire J., Aubert-Foucher E., Herbage D., Mallein-Gérin F
  • «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
    Guerret S., Govignon E., Hartmann D.J., Ronfard V.
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    Plenat F., Vignaud J.M., Guerret-Stocker S., Hartmann D., Duprez K., Duprez A.
  • «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
    Demarchez M., Hartmann D.J., Regnier M., Asselineau D.
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    Begue-Kirn C., Smith A.J., Ruch J.V., Wozney J.M., Purchio A., Hartmann D.J., Lesot H.
  • «Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection. Differentiation 1991, 46, 89-95.»
    Bolmont C., Andujar M., Peyrol S., Grimaud J.A.
  • «Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 1988, 88, 131-140.»
    Andujar M.B., Hartmann D.J., Emonard H., Magloire H.
  • «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
    Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P.
  • «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
    Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A.
  • «Histo-immunolocalisation des collagènes de type I et III humains et murins de cancers humains greffés chez la souris athymique congénitale. C.R. Acad. Sc. Paris 1987, 304, 155-160.»
    Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A.
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    Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M.
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