Anticorps > 20151 - Purified freeze-dried antibody to mouse type I collagen
20151 - Purified freeze-dried antibody to mouse type I collagen
Mouse skin paraffin section
species
Mouse
immunogen
Type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from mouse skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #441h
-
IgG
3.07 mg/mL
pH
7.41
ELISA
Optimal working dilution at 1/40000.
IF
Not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #459g
-
IgG
2.91 mg/mL
pH
7.24
ELISA
Optimal working dilution at 1/30000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #457c
-
IgG
2.25 mg/mL
pH
7.32
ELISA
Optimal working dilution at 1/30000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #474e
-
IgG
4.7 mg/mL
pH
7.30
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin
-
- #470g
-
IgG
5.45 mg/mL
pH
7.32
ELISA
Optimal working dilution at 1/25000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #476g
-
IgG
5.4 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/25000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #496c
-
IgG
3.9 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #497j
-
IgG
1.76 mg/mL
pH
7.32
ELISA
Optimal working dilution at 1/15000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #514c
-
IgG
1.71 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/8000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #510d
-
IgG
2.17 mg/mL
pH
7.31
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #497k
-
IgG
1.91 mg/mL
pH
7.32
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #517j
-
IgG
4.37
pH
7.25
ELISA
Optimal working dilution at 1/2000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #516k
-
IgG
4.48 mg/mL
pH
7.39
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #537c
-
IgG
2.08 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #537c
-
IgG
2.08 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #543c
-
IgG
4.65mg/mL
pH
7.32
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from mouse skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #441h
-
IgG :
3.07 mg/mL
pH :
7.41
ELISA
Optimal working dilution at 1/40000.
IF
Not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #459g
-
IgG :
2.91 mg/mL
pH :
7.24
ELISA
Optimal working dilution at 1/30000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #457c
-
IgG :
2.25 mg/mL
pH :
7.32
ELISA
Optimal working dilution at 1/30000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #474e
-
IgG :
4.7 mg/mL
pH :
7.30
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin
-
- #470g
-
IgG :
5.45 mg/mL
pH :
7.32
ELISA
Optimal working dilution at 1/25000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #476g
-
IgG :
5.4 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/25000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #496c
-
IgG :
3.9 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
- #497j
-
IgG :
1.76 mg/mL
pH :
7.32
ELISA
Optimal working dilution at 1/15000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #514c
-
IgG :
1.71 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/8000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #510d
-
IgG :
2.17 mg/mL
pH :
7.31
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #497k
-
IgG :
1.91 mg/mL
pH :
7.32
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #517j
-
IgG :
4.37
pH :
7.25
ELISA
Optimal working dilution at 1/2000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #516k
-
IgG :
4.48 mg/mL
pH :
7.39
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #537c
-
IgG :
2.08 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #537c
-
IgG :
2.08 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/30 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded mouse skin.
-
- #543c
-
IgG :
4.65mg/mL
pH :
7.32
ELISA
Optimal working dilution at 1/20000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded mouse skin.
-
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Seixas S.I.L., Wajsenzon I.J., Savino W., Quirico-Santos T. - «Host-Donor interactions in healing of human split-thickness skin grafts onto nude mice: in situ hybridization, immunohistochemical, and histochemical studies. Transplantation 1992, 53, 1002-1010. »
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Demarchez M., Hartmann D.J., Regnier M., Asselineau D. - «Effects of dentin proteins, transforming growth factor §1 (TGF§1) and bone morphogenetic protein 2 (BMP2) on the differentiation of odontoblast in vitro. Int. J. Dev. Biol. 1992, 36, 491-503.»
Begue-Kirn C., Smith A.J., Ruch J.V., Wozney J.M., Purchio A., Hartmann D.J., Lesot H. - «Desmin expression in fibroblasts of murine periovular granuloma during liver Schistosoma mansoni infection. Differentiation 1991, 46, 89-95.»
Bolmont C., Andujar M., Peyrol S., Grimaud J.A. - «Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 1988, 88, 131-140.»
Andujar M.B., Hartmann D.J., Emonard H., Magloire H. - «Immunofluorescent localization of collagen types I, III and IV, fibronectin, laminin, and basement membrane proteoglycan in developing mouse skin. Roux's Arch. Dev. Biol. 1987, 196, 295-302.»
Mauger A., Emonard H., Hartmann D.J., Foidart J.M., Sengel P. - «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. - «Histo-immunolocalisation des collagènes de type I et III humains et murins de cancers humains greffés chez la souris athymique congénitale. C.R. Acad. Sc. Paris 1987, 304, 155-160.»
Duprez A., Guerret-Stocker S., Plenat F., Vignaud J.M., Hartmann D., Grimaud J.A. - «Wound-healing of human-skin transplanted onto the nude-mouse. 2. an immunohistological and ultrastructural study of the epidermal basement-membrane zone reconstruction and connective-tissue reorganization. Dev. Biol. 1987, 121, 119-129.»
Demarchez M., Hartmann D.J., Herbage D., Ville G., Prunieras M. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
