Anticorps > 20181 - Purified freeze-dried antibody to sheep type I collagen
20181 - Purified freeze-dried antibody to sheep type I collagen
Sheep skin paraffin section, IHC
species
Sheep
immunogen
Type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish].
Immunogen : Type I collagen extracted from sheep skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/3000 (OD = 0.5)
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #462g
-
IgG
4.65 mg/mL
pH
7.21
ELISA
Optimal working dilution at 1/1000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α1(I) chains and one α2(I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease andidiopathic osteoporosis. [according to R. Dalgleish].
Description
Immunogen : Type I collagen extracted from sheep skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/3000 (OD = 0.5)
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #462g
-
IgG :
4.65 mg/mL
pH :
7.21
ELISA
Optimal working dilution at 1/1000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human skin.
-
