Anticorps > 23151 - Purified freeze-dried antibody to mouse type I procollagen
23151 - Purified freeze-dried antibody to mouse type I procollagen
Mouse skin paraffin section, IHC
species
Mouse
immunogen
Pro Type I collagen
Type I procollagen, synthetized by fibroblasts, is the type III collagen precursor.
Immunogen : Type I collagen extracted from mouse skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/100 000 (OD = 0.5)
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #511g
-
IgG
6.17 mg/mL
pH
7.34
ELISA
Optimal working dilution at 1/80000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
Presentation
Type I procollagen, synthetized by fibroblasts, is the type III collagen precursor.
Description
Immunogen : Type I collagen extracted from mouse skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/100 000 (OD = 0.5)
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #511g
-
IgG :
6.17 mg/mL
pH :
7.34
ELISA
Optimal working dilution at 1/80000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
