Anticorps > 20211 - Purified freeze-dried antibody to human type II collagen
20211 - Purified freeze-dried antibody to human type II collagen
Human cartilage paraffin section, IHC
species
Human
immunogen
Type II collagen
Type II collagen is a fibrillar collagen composed of three identical α1(II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Immunogen : Type II collagen extracted from human cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #472d
-
IgG
3.17
pH
7.40
ELISA
Optimal working dilution at 1/6000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human cartilage.
-
Presentation
Type II collagen is a fibrillar collagen composed of three identical α1(II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Description
Immunogen : Type II collagen extracted from human cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #472d
-
IgG :
3.17
pH :
7.40
ELISA
Optimal working dilution at 1/6000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded human cartilage.
-
References
- «Differences in the intrinsic chondrogenic potential of equine
umbilical cord matrix and cord blood mesenchymal stromal/stem
cells for cartilage regeneration. Scientific Reports volume 8, Article number: 13799 (2018)»
Rakic R., Bourdon B., Demoor M., Maddens S., Saulnier N. and Galéra P. - «Analysis of collagen expression during chondrogenic
induction of human bone marrow mesenchymal stem cells - Biotechnol Lett. - 2011 Oct, 33(10):2091-101.»
Perrier E., Ronziere MC., Bareille R., Pinzano A.,Mallein-Gerin F., Freyria AM. - «"Interstitial Perfusion Culture with Specific Soluble Factors Inhibits Type I Collagen Production from Human Osteoarthritic Chondrocytes in Clinical-Grade Collagen Sponges - PLOS One - 2016 Sept, 1 , 11(9).»
Mayer N., Lopa S., Talò G., Lovati A.B., Pasdeloup M., Riboldi S.A., Moretti M., Mallein-Gerin F. - «"Cartilage-Characteristic Matrix Reconstruction
by Sequential Addition of Soluble Factors During
Expansion of Human Articular Chondrocytes
and Their Cultivation in Collagen Sponges - Tissue engineering Part C - 2011»
Claus S., Mayer N., Aubert-Foucher E., Chajra H, Perrier-Groult E., Lafond J., Piperno M., Damour O., Mallein-Gerin F. - «Comparative phenotypic analysis of articular chondrocytes cultured within type I or type II collagen scaffolds. Tissue Eng Part A. 2009, 15, 1233-1245.»
Freyria AM, Ronzière MC, Cortial D, Galois L, Hartmann D, Herbage D, Mallein-Gerin F. - «Interleukin-6 (IL-6) and/or soluble IL-6 receptor down-regulation of human type II collagen gene expression in articular chondrocytes requires a decrease of Sp1.Sp3 ratio and of the binding activity of both factors to the COL2A1 promoter. J Biol Chem. 2008, 283, 4850-4865.»
Porée B., Kypriotou M, Chadjichristos C, Beauchef G, Renard E, Legendre F, Melin M, Guerret S, Hartmann DJ, Mallein-Gerin F, Pujol JP, Boumediene K, Galéra P. - «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
Guerret S., Govignon E., Hartmann D.J., Ronfard V. - «Electrophoretic and immunochemeical study of collagens from Sepia officinalis cartilage. Biochim. Biophys. Acta 2002, 1572, 77-84.»
Rigo C., Hartmann D.J., Bairati A. - «Control of heterotypic fibril formation by collagen V is determined by chain stoichiometry. J. Biol. Chem. 2001, 276, 24352-24359.»
Chanut-Delalande H., Fichard A., Bernocco S., Garrone R., Hulmes D.J.S., Ruggiero F. - «Bone organotypic culture method: a model for cytocompatibility testing of biomaterials. Cells and Materials 1994, 4, 113-123.»
Anselme K., Lanel B., Gentil C., Hardouin P., Marie P.J., Sigot-Luizard M.F. - «Collagen composition and ultrastructure of the so-called amianthoid fibres in palisaded myofibroblastoma. Ultrastructural and immunohistochemical study. J. Pathol. 1992, 167, 335-340.»
Skalova A., Michal M., Chlumska A., Leivo I. - «Analysis of collagen isotypes in crystalloid structures of salivary gland tumors. Hum. Pathol. 1992, 23, 748-754.»
Skalova A., Leivo I., Michal M. Saksela E. - «The role of fibroblasts in dermal vascularization and remodeling of reconstructed human skin after transplantation onto the nude mouse. Transplantation 1992, 54, 317-326.»
Demarchez M., Hartmann D.J., Regnier M., Asselineau D. - «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
