Anticorps > 20341 - Purified freeze-dried antibody to rat type III collagen
20341 - Purified freeze-dried antibody to rat type III collagen
Rat skin paraffin section, IHC
species
Rat
immunogen
Type III collagen
Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]
Immunogen : Type III collagen extracted from rat skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA)
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Cross-reactivity with mouse type III collagen.
- #357c
-
IgG
2.6 mg/mL
pH
7.4
ELISA
Optimal working dilution at 1/3000.
IF
IF not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #357g
-
IgG
3.04
pH
7.33
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
- #515d
-
IgG
2.98 mg/mL
pH
7.27
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded rat skin.
-
- #557c
-
IgG
2.79 mg/mL
pH
7.43
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
Presentation
Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]
Description
Immunogen : Type III collagen extracted from rat skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA)
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Specificity
Cross-reactivity with mouse type III collagen.
Lots
- #357c
-
IgG :
2.6 mg/mL
pH :
7.4
ELISA
Optimal working dilution at 1/3000.
IF
IF not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.
-
- #357g
-
IgG :
3.04
pH :
7.33
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
- #515d
-
IgG :
2.98 mg/mL
pH :
7.27
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded rat skin.
-
- #557c
-
IgG :
2.79 mg/mL
pH :
7.43
ELISA
Optimal working dilution at 1/4000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
References
- «"De novo generation in an in vivo rat model and biomechanical characterization of autologous transplants for ligament and tendon reconstruction. Clinical Biomechanics. 2017 Dec 14;52:33-40. "»
Soubeyranda M., Laemmel E., Maurel N., Diop A., Lazure T., Duranteau J., Vicaut E. - «Isolation and characterization of rat alveolar bone cells. Cell Mol. Biol. 1991, 37, 509-517.»
Bouvier M., Couble M.L., Hartmann D.J., Magloire H. - «Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs. J. Biol. Chem. 1990, 265, 6286-6290.»
Breen E., Falco V.M., Absher M., Cutroneo K.R. - «Ultrastructural and immunocytochemical study of bone-derived cells cultured in three-dimensional matrices : influence of chondroitin-4 sulfate on mineralization. Differentiation 1990, 45, 128-137.»
Bouvier M., Couble M.L., Hartmann D.J., Gauthier J.P., Magloire H. - «Immunohistochemical study of the biological fate of a subcutaneous bovine collagen implant in rat. Histochemistry 1989, 91, 177-184.»
Vialle-Presles M.J., Hartmann D.J., Franc S., Herbage D. - «Differential immunohistochemical localization of cytokeratins and collagen types I and III in experimentally-induced cirrhosis. J. Pathol. 1989, 159, 151-158.»
Al Adnani M.S. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
