20341 - Purified freeze-dried antibody to rat type III collagen

Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]

Immunogen : Type III collagen extracted from rat skin.

Host : Rabbit.

Polyclonal antibodies purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA)

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

Cross-reactivity with mouse type III collagen.

• #357c

  • IgG : 2.6 mg/mL pH : 7.4

    ELISA Optimal working dilution at 1/3000. IF IF not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

• #357g

  • IgG : 3.04 pH : 7.33

    ELISA Optimal working dilution at 1/4000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.

• #515d

  • IgG : 2.98 mg/mL pH : 7.27

    ELISA Optimal working dilution at 1/4000. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded rat skin.