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Anticorps > 24951 - Purified freeze-dried antibody to mouse fibronectin

24951 - Purified freeze-dried antibody to mouse fibronectin



Mouse intestine, IHC(p)


species

Mouse



immunogen

Fibronectin




Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]

Immunogen : Fibronectin extracted from mouse plasma.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #403e
  • #429k
  • #506c

Presentation

Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]



Description

Immunogen : Fibronectin extracted from mouse plasma.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 


Use

Applications

IF, IHC, ELISA, SP(RIA).

 

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.


Lots

  • #403e
    • IgG :

      2.76 mg/mL



      pH :

      7.56

      ELISA


      Optimal working dilution at 1/50000.


      IF


      Not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

  • #429k
    • IgG :

      4.03 mg/mL



      pH :

      7.32

      ELISA


      Optimal working dilution at 1/60000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

  • #506c
    • IgG :

      3.8 mg/mL



      pH :

      7.29

      ELISA


      Optimal working dilution at 1/100000.


      IF


      Optimal working dilution not tested with our current protocol.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.