24951 - Purified freeze-dried antibody to mouse fibronectin

Fibronectin is  a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. [according to RefSeq]

Immunogen : Fibronectin extracted from mouse plasma.

Host : Rabbit.

Polyclonal antibodies purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Liquid bulk of 5 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #526c

  • IgG : 3.53 mg/mL pH : 7.34

    ELISA Optimal working dilution at 1/100000. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse skin.

• #540c

  • IgG : 3.49 mg/mL pH : 7.46

    ELISA Optimal working dilution at 1/100 000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

• #403e

  • IgG : 2.76 mg/mL pH : 7.56

    ELISA Optimal working dilution at 1/50000. IF Not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

• #429k

  • IgG : 4.03 mg/mL pH : 7.32

    ELISA Optimal working dilution at 1/60000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

• #506c

  • IgG : 3.8 mg/mL pH : 7.29

    ELISA Optimal working dilution at 1/100000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

• #526d

  • IgG : 3.53 mg/mL pH : 7.34

    ELISA Optimal working dilution at 1/20000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.

• #535c

  • IgG : 3.85 mg/mL pH : 7.04

    ELISA Optimal working dilution at 1/110000. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded mouse intestine.