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Anticorps > 20171T - Purified freeze-dried antibody to tuna fish type I collagen

20171T - Purified freeze-dried antibody to tuna fish type I collagen



Tuna skin paraffin section, IHC


species

Fishes



immunogen

Type I collagen




Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…).

Immunogen : Type I collagen extracted from tuna fish skin.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IFA, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Share your experience with this antibody in your specific application

  • #330g
  • #350i

Presentation

Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…).



Description

Immunogen : Type I collagen extracted from tuna fish skin.

Host : Rabbit.

 

Polyclonal antibodies purified by chromatography.

 

For research only.



Format

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.


Stability

Unreconstituted

24 months at -20°C.

 

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.


Use

Applications

IFA, IHC, ELISA, IB/WB, SP(RIA).

 

Working dilutions

IF ≥ 1/40

IHC ≥ 1/500

ELISA ≥ 1/2000 (OD = 0.5)

Optimal dilutions should be determined by the end user.


Lots

  • #330g
    • IgG :

      2,3



      pH :

      7,3

      ELISA


      Optimal working dilution not tested.


      IF


      With fluorescein anti-rabbit IgG conjugate, uoptimal working dilution at 1/160 with frozen tuna fish skin.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/2000 with fixed paraffin-embedded tuna fish skin.

  • #350i
    • IgG :

      4.0 mg/mL



      pH :

      7.3

      ELISA


      Optimal working dilution at 1/2000.


      IF


      Optimal dilution not tested.


      IHC


      Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded tuna fish skin.