Antibody > 27071-1 - Purified freeze-dried antibody to tuna fish type I collagen
27071-1 - Purified freeze-dried antibody to tuna fish type I collagen
Tuna skin paraffin section, IHC
species
Fishes
immunogen
Type I collagen
Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…).
Immunogen : Type I collagen extracted from tuna fish skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IFA, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
- #330g
-
IgG
2,3 mg/mL
pH
7,3
ELISA
Optimal working dilution not tested.
IF
With fluorescein anti-rabbit IgG conjugate, uoptimal working dilution at 1/160 with frozen tuna fish skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/2000 with fixed paraffin-embedded tuna fish skin.
-
- #350i
-
IgG
4.0 mg/mL
pH
7.3
ELISA
Optimal working dilution at 1/2000.
IF
Optimal dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded tuna fish skin.
-
- #522c
-
IgG
7.28 mg/mL
pH
7.33
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded tuna fish skin.
-
Presentation
Type I collagen, belonging to the fibrillar collagens group, is composed of two identical α1(I) chains and one α2(I) chains [α1(I)]2[α(I)]. Type I collagen is a major collagen found in several connective tissues (skin, bone, ligament, tendon, heart…).
Description
Immunogen : Type I collagen extracted from tuna fish skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL diH2O and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IFA, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
Lots
- #330g
-
IgG :
2,3
pH :
7,3
ELISA
Optimal working dilution not tested.
IF
With fluorescein anti-rabbit IgG conjugate, uoptimal working dilution at 1/160 with frozen tuna fish skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/2000 with fixed paraffin-embedded tuna fish skin.
-
- #350i
-
IgG :
4.0 mg/mL
pH :
7.3
ELISA
Optimal working dilution at 1/2000.
IF
Optimal dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5%hyaluronidase pretreatment, optimal working dilution at 1/4000 on fixed paraffin-embedded tuna fish skin.
-
- #522c
-
IgG :
7.28 mg/mL
pH :
7.33
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/2000 on fixed paraffin-embedded tuna fish skin.
-
