
Anticorps > 20191 - Purified freeze-dried antibody to porcine type I collagen
20191 - Purified freeze-dried antibody to porcine type I collagen
Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Immunogen : Type I collagen extracted from porcine tendon.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #311h
-
IgG
5,5 mg/mL
pH
7,7
ELISA
Optimal working dilution not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen porcine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment (only on paraffin sections), optimal working dilution at 1/1000 on fixed frozen or paraffin-embedded porcine skin.
-
- #311g
-
IgG
5,5 mg/mL
pH
7,7
ELISA
Optimal working dilution at 1/200.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
-
Presentation
Type I collagen is a fibrillar collagen composed of two identical chain α 1 (I) chains and one α 2 (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]
Description
Immunogen : Type I collagen extracted from porcine tendon.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #311h
-
IgG :
5,5
pH :
7,7
ELISA
Optimal working dilution not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen porcine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment (only on paraffin sections), optimal working dilution at 1/1000 on fixed frozen or paraffin-embedded porcine skin.
-
- #311g
-
IgG :
5,5
pH :
7,7
ELISA
Optimal working dilution at 1/200.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.
-
References
- «Osteoblastic differentiation of Wharton's jelly biopsies and its mesenchymal stromal cells after xeno-free culture. Plastic and Reconstructive Surgery. Plast Reconstr Surg. 2014 Jul;134(1):59e-69e.»
Mueller A., Forraz N., Gueven S., Atzenia G., Degoul O., Pagnon-Minot A., Hartmann D., Martin I., Scherberich A., McGuckin C. - «"In vitro cytocompatibility of porcine type I atelocollagen crosslinked by oxidized glycogen. Biomaterials 2002, 23, 1503-1510."»
Rousseau C.F, Gagnieu C.H.