20191 - Purified freeze-dried antibody to porcine type I collagen

Type I collagen is a fibrillar collagen composed of two identical chain α ₁ (I) chains and one α ₂ (I). Type I collagen is found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. [according to R. Dalgleish]

Immunogen : Type I collagen extracted from porcine tendon.

Host : Rabbit.

Polyclonal antibody purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #311h

  • IgG 5,5 mg/mL pH 7,7

    ELISA Optimal working dilution not tested with our current protocol. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen porcine skin. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment (only on paraffin sections), optimal working dilution at 1/1000 on fixed frozen or paraffin-embedded porcine skin.

• #311g

  • IgG 5,5 mg/mL pH 7,7

    ELISA Optimal working dilution at 1/200. IF Optimal working dilution not tested. IHC Immunoperoxidase technique with the Envision / Dako kit, hyaluronidase 0.5% pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded human skin.