20491 - Purified freeze-dried antibody to porcine type IV collagen

Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]

 Immunogen : Type IV collagen extracted from porcine kidney tissues.

Host : Rabbit.

Polyclonal antibodies purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles. 

Applications

IF, IHC, ELISA, IB/WB, SP(RIA).

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #312k

  • IgG 1,1 mg/mL pH 7,5

    ELISA ELISA not tested with our current protocol. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen porcine skin, liver. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatement, optimal working dilution at 1/1000 on fixed paraffin-embedded porcine skin.

• #312l

  • IgG 1,1 mg/mL pH 7,5

    ELISA Optimal working dilution not tested. IF With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen porcine skin, liver. IHC Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatement, optimal working dilution at 1/1000 on fixed paraffin-embedded porcine skin.