Anticorps > 20221 - Purified freeze-dried antibody to bovine type II collagen
20221 - Purified freeze-dried antibody to bovine type II collagen
Bovine cartilage paraffin section, IHC
species
Bovine
immunogen
Type II collagen
Type II collagen is a fibrillar collagen composed of three identical α1(II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Immunogen : Type II collagen extracted from bovine cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #321j
-
IgG
8,5 mg/mL
pH
7,6
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine cartilage tissues.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine cartilage.
-
- #321n
-
IgG
3.16
pH
7.3
ELISA
Optimal working dilution at 1/10 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine cartilage.
-
Presentation
Type II collagen is a fibrillar collagen composed of three identical α1(II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Description
Immunogen : Type II collagen extracted from bovine cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #321j
-
IgG :
8,5
pH :
7,6
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine cartilage tissues.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine cartilage.
-
- #321n
-
IgG :
3.16
pH :
7.3
ELISA
Optimal working dilution at 1/10 000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded bovine cartilage.
-
References
- «Influence of medium composition, static and stirred conditions on the proliferation of and matrix protein expression of bovine articular chondrocytes cultures in 3-D collagen scaffold. Biomaterial 2004, 25, 687-697.»
Freyria A.M., Cortial D., Ronzière M.C., Guerret S., Herbage D. - «Immunohistochemical study of collagens of the extracellular matrix in cartilage of Sepia officinalis. Eur. J. Histochem. 1999, 43, 211-225.»
Bairati A., Comazzi M., Gioria M., Hartmann D.J., Leone F., Rigo C. - «Analysis of types I, II, III, IX and XI collagens synthesized by fetal bovine chondrocytes in high-density culture. Osteoarthritis Cart. 1997, 5, 205-214.»
Ronziere M.C., Farjanel J., Freyria A.M., Hartmann D.J., Herbage D. - «Composition and organization of the collagen network produced by fetal bovine chondrocytes cultured at high density. J. Histochem. Cytochem. 1993, 41, 867-875.»
Ruggiero F., Petit B., Ronzière M.C., Farjanel J., Hartmann D.J., Herbage D. - «Ultrastuctural organization of type XI collagen in fetal bovine epiphyseal cartilage. Histochemistry 1993, 100, 231-239.»
Petit B., Ronzière M.C., Hartmann D.J., Herbage D. - «Comparative analysis of collagens solubilized from human foetal, and normal and osteoarthritic adult articular cartilage, with emphasis on type VI collagen. Biochim. Biophys. Acta 1990, 1038, 222-230.»
Ronzière M.C., Ricard-Blum S., Tiollier J., Hartmann D.J., Garrone R., Herbage D. - «Physiological behaviour of rabbit articular chondrocytes immobilized in a calcium alginate gel. In : Physiology of immobilized cells, J.A.M.de Bont, Tramper J., Hamer G., Harder W., Mattiasson B. Eds, Elsevier Science PublImhers B.V., Amsterdam, 1990.»
Guyot J.B., Tamponnet C., Lièvremont M. - «Problems in the immunolocalization of type IX collagen in fetal calf cartilage using a monoclonal antibody. Connec. Tissue Res. 1989, 18, 277-292.»
Vilamitjana J., Barge A., Julliard A.K., Herbage D., Baltz T., Garrone R., Harmand M-F. - «Rabbit articular chondrocytes : an in vitro model for studying the effect of sodium auro-thiopropanol sulfonate on proliferation kinectics, type II collagen phenotype and mitochondrial activity. Fundam. Clin.Pharmacol. 1988, 2,57-67.»
Ronot X., Hainque B., ChrImten M.O., Froger B., Hartmann D.J., Adolphe M., Lechat P. - «The interstitial matrix of human carcinomas and sarcomas transplanted to the nude mouse : immunolocalization of some human and murine components. Cell. Mol. Biol. 1987, 33, 647-654.»
Duprez A., Guerret S., Vignaud J.M., Plenat F., Hartmann D.J., Grimaud J.A. - «"Collagen expression during teratocarcinoma cell line-induced endochondral bone tumor. J. Histochem. Cytochem. 1986, 34, 835-839."»
"Boy-Lefevre M.L., Nefussi J.R., Paulin D., Hartmann D.J., Ricard Blum S., Herbage D., Forest N. " - «Collagen heterogeneity in bovine and human cartilage. In : Osteoarthritis-Current clinical and fundamental problems, Peyron J.G. Ed. Primard, Paris, 1985, 123-133.»
Ricard-Blum S., Tiollier J., Dodille M.P., Hartmann D.J., Magloire H., Garrone R., Ville G., Herbage D. - «Biochemical properties and immunolocalization of minor collagens in foetal calf cartilage. FEBS Letters 1982, 146, 343-347.»
Ricard-Blum S., Hartmann D.J., Herbage D., Payen-Meyran C., Ville G.
