Anticorps > 23111 - Purified freeze-dried antibody to human type I pro-collagen
23111 - Purified freeze-dried antibody to human type I pro-collagen
species
Human
immunogen
Pro Type I collagen
Type I procollagen, synthetized by fibroblasts, is the type III collagen precursor.
Immunogen : Type I collagen extracted from human skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #119i
-
IgG
1.36 mg/mL
pH
7.5
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/100 on fixed human fibroblasts.
-
- #561g
-
IgG
1.5mg/mL
pH
7.30
ELISA
Optimal working dilution at 1/60 000.
IF
Not tested with our current protocol.
IHC
Not tested with our current protocol.
-
Presentation
Type I procollagen, synthetized by fibroblasts, is the type III collagen precursor.
Description
Immunogen : Type I collagen extracted from human skin.
Host : Rabbit.
Polyclonal antibodies purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA.
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #119i
-
IgG :
1.36 mg/mL
pH :
7.5
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/100 on fixed human fibroblasts.
-
- #561g
-
IgG :
1.5mg/mL
pH :
7.30
ELISA
Optimal working dilution at 1/60 000.
IF
Not tested with our current protocol.
IHC
Not tested with our current protocol.
-
