23111 - Purified freeze-dried antibody to human type I pro-collagen

Type I procollagen, synthetized by fibroblasts, is the type III collagen precursor.

Immunogen : Type I collagen extracted from human skin.

Host : Rabbit.

Polyclonal antibodies purified by chromatography.

For research only.

Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.

Liquid bulk of 5 mL and 10 mL. Aliquoted and stored at -20°C.

Unreconstituted

24 months at -20°C.

Reconstituted

Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.

Applications

IF, IHC, ELISA.

Working dilutions

ELISA ≥ 1/2000 (OD = 0.5)

IF ≥ 1/40

IHC ≥ 1/500

Optimal dilutions should be determined by the end user.

• #119i

  • IgG : 1.36 mg/mL pH : 7.5

    ELISA Optimal working dilution at 1/10000. IF Optimal working dilution not tested with our current protocol. IHC Immunoperoxidase technique with the Envision / Dako kit, optimal working dilution at 1/100 on fixed human fibroblasts.

• #561g

  • IgG : 1.5mg/mL pH : 7.30

    ELISA Optimal working dilution at 1/60 000. IF Not tested with our current protocol. IHC Not tested with our current protocol.