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Histologie / Coloration



L'histologie permet de visualiser et d'analyser la morphologie d'un organe et de décrire les structures spécifiques des tissus/cellules.




Novotec réalise l'inclusion des tissus en paraffine, en milieu de congélation ou en résine (Epoxy ou MMA), la coupe des blocs et la coloration des coupes obtenues. En fonction des projets, des colorations appropriées sont proposées. La prestation peut également comprendre l'observation et l'interprétation des résultats par la rédaction d'un rapport détaillé, illustré par des planches photos.

Différenciation ostéogénique - Coloration Von Kossa Poisson zèbre - Coloration MTG

 

 

 

 

 

 

 

 

 

 

 

 

 

Types d'échantillons : tissus mous (peau, foie, rein, cerveau, rate, ...), tissus durs (os, cartilage), cellules en gel tridimentionnel, biomatériaux (naturels et polymères).  

 

Colorations standards :

  • Hematoxyline- Eosine -Safran (HES)
  • Trichrome de Masson
  • Trichrome de Masson Goldner (MTG).

Foie - Coloration PAS  

Colorations spécifiques :

  • Collagènes : Rouge de picrosirius, Gordon Sweet
  • Elastine : Van Gieson (Verhoeff)
  • Glycoaminoglycanes : Safranine O (SO), Bleu Alcian
  • Glycogène : Periodic Acid Schiff (PAS), Bleu de Toluidine
  • Lipides : Red Oil
  • Inflammation : May Grünwald Giemsa (MGG)
  • Fer : Perl's
  • ...




References

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    Laurent R., Nallet A., De Billy B., Obert L., Nicod L., Meyer C., Layrolle P., Zwetyenga N., Gindraux F.
  • «Analysis of collagen expression during chondrogenic induction of human bone marrow mesenchymal stem cells - Biotechnol Lett. - 2011 Oct, 33(10):2091-101.»
    Perrier E., Ronziere MC., Bareille R., Pinzano A.,Mallein-Gerin F., Freyria AM.
  • «Storage and qualification of viable intact human amniotic graft and technology transfer to a tissue bank. Cell Tissue Bank. 2014 Jun;15(2):267-75.»
    Laurent R., Nallet A., Obert L., Nicod L., Gindraux F.
  • «Osteoblastic differentiation of Wharton's jelly biopsies and its mesenchymal stromal cells after xeno-free culture. Plastic and Reconstructive Surgery. Plast Reconstr Surg. 2014 Jul;134(1):59e-69e.»
    Mueller A., Forraz N., Gueven S., Atzenia G., Degoul O., Pagnon-Minot A., Hartmann D., Martin I., Scherberich A., McGuckin C.
  • «"Cartilage-Characteristic Matrix Reconstruction by Sequential Addition of Soluble Factors During Expansion of Human Articular Chondrocytes and Their Cultivation in Collagen Sponges - Tissue engineering Part C - 2011»
    Claus S., Mayer N., Aubert-Foucher E., Chajra H, Perrier-Groult E., Lafond J., Piperno M., Damour O., Mallein-Gerin F.
  • «In situ Hybridization to evaluate the wound healing process of a bilayered living cell-based construct (Apligraf®) in a nude mice model - 2010 june, TERMIS-EU, Galway, Ireland.»
    Rousseau C. and Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.»
    Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V.
  • «Hyperphosphataemia sensitizes renally impaired rats to the profibrotic effects of gadodiamide. Br J Pharmacol, 2012 Feb;165(4b):1151-1162.»
    Fretellier N., Idée J.M., Bruneval P., Guerret S., Daubiné F., Jestin G., Factor C., Poveda N., Dencausse A., Massicot F., Laprévote O., Mandet C., Bouzian N., Port M., Corot C.
  • «Comparative In Vivo Dissociation of Gadolinium Chelates in Renally Impaired Rats: A Relaxometry Study. Invest Radiol. 2011, 46, 292-300.»
    Fretellier N., Idée J.M., Dencausse A., Karroum O., Guerret S., Poveda N., Jestin G., Factor C., Raynal I., Zamia P., Port M., Corot C.
  • «Clinical, biological, and skin histopathologic effects of ionic macrocyclic and nonionic linear gadolinium chelates in a rat model of nephrogenic systemic fibrosis. Invest Radiol. 2011, 46, 85-93.»
    Fretellier N., Idée J.M., Guerret S., Hollenbeck C., Hartmann D., González W., Robic C., Port M., Corot C.
  • «Protein-losing enteropathy of non neoplastic origin in the dog: a retrospective study of 34 cases. Schweiz Arch Tierheilkd. 2010, 152, 141-146.»
    Lecoindre P, Chevallier M, Guerret S
  • «Analyse histologique des bois de chevreuil et réfléxion autour d'un modèle innovant de réparation osseuse. Bio recontruction de l'os à la peau. Sept 2009, 41-57.»
    Gindraux F., Obert L.
  • «Rise in gamma interferon expression during resolution of duck hepatitis B virus infection. J. Gen.Virol. 2006, 87, 3225-3232.»
    Narayan R., Buronfosse T., Schultz U., Chevallier-Gueyron P., Guerret S., Chevallier M., Saade F., Ndeboko B., Trepo C., Zoulim F., Cova L.
  • «Complete unilateral ureteral obstruction in the fetal lamb. Part II: Long-term outcomes of renal tissue development. J Urol. 2006, 4, 1548-1558.»
    Mure P.Y., Gelas T, Dijoud F, Guerret S, Benchaib M, Hartmann DJ, Mouriquand P.
  • «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
    Guerret S., Govignon E., Hartmann D.J., Ronfard V.