Antibody > 20241 - Purified freeze-dried antibody to rat type II collagen
20241 - Purified freeze-dried antibody to rat type II collagen
Rat cartilage paraffin section, IHC
species
Rat
immunogen
Type II collagen
Type II collagen is a fibrillar collagen composed of three identical α1 (II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Immunogen : Native type II collagen extracted from rat cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #349g
-
IgG
4.2 mg/mL
pH
7.6
ELISA
Optimal working dilution at 1/800.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
Presentation
Type II collagen is a fibrillar collagen composed of three identical α1 (II) chains. The type II collagen is found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. [according to RefSeq]
Description
Immunogen : Native type II collagen extracted from rat cartilage.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #349g
-
IgG :
4.2 mg/mL
pH :
7.6
ELISA
Optimal working dilution at 1/800.
IF
Optimal working dilution not tested.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/1000 on fixed paraffin-embedded rat skin.
-
References
- «"De novo generation in an in vivo rat model and biomechanical characterization of autologous transplants for ligament and tendon reconstruction. Clinical Biomechanics. 2017 Dec 14;52:33-40. "»
Soubeyranda M., Laemmel E., Maurel N., Diop A., Lazure T., Duranteau J., Vicaut E. - «Collagen immunotyping in human liver : Light and electron microscope study. J. Histochem. Cytochem. 1980, 28, 1145-1156.»
Grimaud J.A., Druguet M., Peyrol S., Chevalier O., Herbage D., El Badrawy N.
