Antibody > 20321 - Purified freeze-dried antibody to bovine type III collagen
20321 - Purified freeze-dried antibody to bovine type III collagen
Bovine skin paraffin section, IHC
species
Bovine
immunogen
Type III collagen
Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]
Immunogen : Type III collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
- #293o
-
IgG
10
pH
7,3
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine skin.
-
Presentation
Type III collagen is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. [according to R. Dalgleish]
Description
Immunogen : Type III collagen extracted from bovine skin.
Host : Rabbit.
Polyclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1 mL vials. Reconstitute with 0.1 mL distilled water and store aliquots at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, SP(RIA).
Working dilutions
ELISA ≥ 1/2000 (OD = 0.5)
IF ≥ 1/40
IHC ≥ 1/500
Optimal dilutions should be determined by the end user.
Lots
- #293o
-
IgG :
10
pH :
7,3
ELISA
ELISA not tested with our current protocol.
IF
With fluorescein anti-rabbit IgG conjugate, optimal working dilution at 1/40 on frozen bovine skin.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/500 on fixed paraffin-embedded bovine skin.
-
References
- «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
Guerret S., Govignon E., Hartmann D.J., Ronfard V. - «Structural and compositional divergencies in the extracellular matrix encountered by neural crest cells in the white mutant axolotl embryo. Development 1990, 109, 533-551.»
Perris R., Löfberg J., Fällström C., Von Boxberg Y., Olsson L., Newgreen D.F.
