Antibody > 60415 - Purified freeze-dried antibody to human type IV collagen
60415 - Purified freeze-dried antibody to human type IV collagen
Human skin paraffin section, IHC
species
Human
immunogen
Type IV collagen
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Immunogen : Purified type IV collagen extracted from human placenta.
Source material : Mouse (Nude strain) ascites.
Isotype : IgG1k
Monoclonal antibody purified by chromatography.
For research only.
Purified, freeze-dried antibody in 0.1mL vials. Reconstitute with 0.1mL distilled water and store at -20°C.
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Applications
IF, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
24D1
- #451g
-
IgG
0.57
pH
7.43
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/16000 on fixed paraffin-embedded human skin.
-
Presentation
Type IV collagen is a sheet-forming type of collagen found in basement membranes where it serves a critical scaffolding function. In mammals, six proteins make up the type IV collagen family with the distribution of family members being tissue-specific. Structurally, three collagen IV alpha chains assemble to form a flexible heterotrimeric protomer that can self-assemble into network polymers. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV α3 chain. Mutations in COL4A5 are associated with Alport syndrome. [according to RefSeq]
Description
Immunogen : Purified type IV collagen extracted from human placenta.
Source material : Mouse (Nude strain) ascites.
Isotype : IgG1k
Monoclonal antibody purified by chromatography.
For research only.
Format
Purified, freeze-dried antibody in 0.1mL vials. Reconstitute with 0.1mL distilled water and store at -20°C.
Stability
Unreconstituted
24 months at -20°C.
Reconstituted
Before use aliquot and store at -20°C (6 months). Avoid repeated freeze/thaw cycles.
Use
Applications
IF, IHC, ELISA, IB/WB, SP(RIA).
Working dilutions
IF ≥ 1/40
IHC ≥ 1/500
ELISA ≥ 1/2000 (OD = 0.5)
Optimal dilutions should be determined by the end user.
Clone
24D1
Lots
- #451g
-
IgG :
0.57
pH :
7.43
ELISA
Optimal working dilution at 1/10000.
IF
Optimal working dilution not tested with our current protocol.
IHC
Immunoperoxidase technique with the Envision / Dako kit, 0.5% hyaluronidase pretreatment, optimal working dilution at 1/16000 on fixed paraffin-embedded human skin.
-
References
- «Short term integration on human bilayered living skin substitute (Apligraf®) grafted onto nude mice - 19th Annual Meeting of the Wound Healing Society with SAWC (Symposium on Advanced Wound Care) 2009 April 26-29, Gaylord Texan Resort and Convention Center, Dallas, Texas.»
Rousseau C., Thillou F., Pagnon-Minot A., Guerret S., Hartmann D.J, Ronfard V. - «Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg. 2003, 11, 35-45.»
Guerret S., Govignon E., Hartmann D.J., Ronfard V.
